Park Yong Sook, Park Seung Won, Suk Jong Sik, Nam Taik Kyun
Department of Neurosurgery, Chung-Ang University College of Medicine, 65-207 Hangang-ro 3, Yong San gu, Seoul, South Korea.
Childs Nerv Syst. 2011 Jun;27(6):903-10. doi: 10.1007/s00381-011-1398-9. Epub 2011 Feb 1.
Previous animal models of obstructive hydrocephalus that was frequently combined with subarachnoid inflammation are not suitable for investigating cerebrospinal fluid (CSF) flow between ventricles and cisterns in obstructive hydrocephalus. In this study, we attempted to develop a new animal model for obstructive hydrocephalus in rats sparing subarachnoid space using N-butyl cyanoacrylate (NBCA).
Hydrocephalus was induced in adult male Sprague-Dawley rats with NBCA (n=15) or with kaolin (n=10). For the NBCA model, a silicone tube was inserted through the foramen of Magendie into the fourth ventricle, into which 20 μL of a mixture of NBCA and ethiodized oil was injected. For the kaolin model, 100 μL of 20% kaolin solution was injected into the cistern magna. The rats in the NBCA and kaolin groups were sacrificed 3 and 21 days after surgery, respectively.
Eleven rats in the NBCA group developed hydrocephalus (73.3%), with a 13.3% mortality rate. The kaolin group showed hydrocephalus in eight rats (80%), with a 20% mortality rate. The mean Evans' indices were 0.37 ± 0.02, 0.45 ± 0.04, and 0.53 ± 0.09 in the control, NBCA, and kaolin groups, respectively (p < 0.05). There was no remarkable arachnoid adhesion or inflammatory change of the ventricular wall in the NBCA group.
The NBCA model seems to be a useful animal model for acute obstructive hydrocephalus with preserved subarachnoid CSF pathway. This model can be useful for studying CSF flow between ventricles and cisterns.
以往的梗阻性脑积水动物模型常伴有蛛网膜下腔炎症，不适用于研究梗阻性脑积水时脑室与脑池之间的脑脊液（CSF）流动。在本研究中，我们试图使用氰基丙烯酸正丁酯（NBCA）开发一种新的大鼠梗阻性脑积水动物模型，该模型可保留蛛网膜下腔。
成年雄性Sprague-Dawley大鼠分别用NBCA（n = 15）或高岭土（n = 10）诱导脑积水。对于NBCA模型，将硅胶管经马根迪孔插入第四脑室，向其中注入20 μL NBCA与碘化油的混合物。对于高岭土模型，将100 μL 20%高岭土溶液注入枕大池。NBCA组和高岭土组的大鼠分别在术后3天和21天处死。
NBCA组11只大鼠发生脑积水（73.3%），死亡率为13.3%。高岭土组8只大鼠出现脑积水（80%），死亡率为20%。对照组、NBCA组和高岭土组的平均伊文氏指数分别为0.37±0.02、0.45±0.04和0.53±0.09（p<0.05）。NBCA组未观察到明显的蛛网膜粘连或脑室壁炎症改变。
NBCA模型似乎是一种有用的急性梗阻性脑积水动物模型，其蛛网膜下腔CSF通路保留。该模型可用于研究脑室与脑池之间的CSF流动。
