Shaolin Z, Zhanxiang W, Hao X, Feifei Z, Caiquan H, Donghan C, Jianfeng B, Feng L, Shanghang S
Wang Zhanxiang, Department of Neurosurgery, The First Affiliated Hospital of Xiamen University, No. 55 ZhenHai Road, Xiamen 361003, China, phone: +86 5922139815, fax: +86 5922139315, e-mail:
Folia Neuropathol. 2015;53(1):60-8. doi: 10.5114/fn.2015.49975.
Hydrocephalus is a common neurological disease in humans, but a uniform and particularly effective hydrocephalic animal model amenable to proper appraisal and deep study has not yet been established. In this study, we attempted to construct a high-efficiency model of hydrocephalus via intraventricular kaolin injection. Adult male Sprague-Dawley rats were randomly divided into 2 groups: the control group (n = 15) and the experimental group (n = 30). Kaolin was injected into the lateral ventricle of experimental animals. Control rats underwent the same procedure but received sterile saline injection instead of kaolin. All animals with kaolin injection into the lateral ventricle developed hydrocephalus according to magnetic resonance imaging (MRI) results (success rate up to 100%). Also, the Morris water maze (MWM) test demonstrated disturbed spatial learning and memory. Furthermore, there were significant differences between groups with respect to the histological changes in the periventricular tissue. Our results indicate that experimental hydrocephalus induced by lateral ventricle injection of kaolin in adult rats is feasible and may be widely used.
脑积水是人类常见的神经系统疾病，但尚未建立一种适用于正确评估和深入研究的统一且特别有效的脑积水动物模型。在本研究中，我们试图通过脑室内注射高岭土构建一种高效的脑积水模型。成年雄性Sprague-Dawley大鼠被随机分为2组：对照组（n = 15）和实验组（n = 30）。将高岭土注入实验动物的侧脑室。对照大鼠接受相同的操作，但注射无菌生理盐水而非高岭土。根据磁共振成像（MRI）结果，所有侧脑室注射高岭土的动物均发生了脑积水（成功率高达100%）。此外，莫里斯水迷宫（MWM）试验表明空间学习和记忆受到干扰。此外，脑室周围组织的组织学变化在各组之间存在显著差异。我们的结果表明，成年大鼠侧脑室注射高岭土诱导的实验性脑积水是可行的，可能会得到广泛应用。
