使用无蛋白培养基连续运行的哺乳动物细胞培养过程的性能特征。
Performance characteristics of mammalian cell culture process operating continuously with protein-free medium.
作者信息
Bliem R, Oakley R, Matsuoka K, Taiariol V
机构信息
Bio-Response, Inc., Hayward, CA 94545.
出版信息
Appl Biochem Biotechnol. 1990 Dec;26(3):217-29. doi: 10.1007/BF02921503.
PMID:2128677
Abstract
In this communication we briefly describe a continuous industrial process for the production of Tissue Plasminogen Activator (tPA), operating with a standard, basal medium (DME/F12). Process data of two culture runs has been chosen to present a performance profile over a production period of more than 3 mo. Developmental aspects of glucose utilization (as a process parameter) are briefly discussed.
摘要
在本通讯中，我们简要描述了一种用于生产组织型纤溶酶原激活剂（tPA）的连续工业生产工艺，该工艺使用标准基础培养基（DME/F12）运行。我们选择了两次培养运行的过程数据，以展示超过3个月生产期的性能概况。简要讨论了葡萄糖利用（作为一个工艺参数）的发展情况。
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