Comparison of the optimal culture conditions for cell growth and tissue plasminogen activator production by human embryo lung cells on microcarriers.

Optimization of culture conditions such as the dissolved O2 (DO) concentration, temperature and pH was attempted regarding both cell growth and the production of tissue plasminogen activator (TPA) in a microcarrier cell culture of human embryo lung cells. The growth rate was suppressed at a DO concentration below 30% saturation. From the pH range 7.2-7.6, both the specific growth rate and maximal cell concentration decreased. At a lower temperature than 37 degrees C, although both the specific growth rate and the maximal cell concentration decreased, the cell concentration was maintained for a longer time during the production period, high TPA productivity being maintained. As the optimal conditions for culture growth, a DO concentration of 30% saturation or over, temperature of 37 degrees C and pH of 7.4 are recommended. However, for TPA production after cell culture growth, the DO concentration should be in the range 20-30% O2 saturation, and the temperature and pH should be lowered to 33 degrees C and 6.8, respectively.