Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, Japan.
Biotechnol Lett. 2011 Jun;33(6):1133-8. doi: 10.1007/s10529-011-0544-5. Epub 2011 Feb 2.
We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATP-regenerating bioprocess for various ATP-hydrolyzing bioproductions.
我们从表达 delta-视紫红质(一种新型的光驱动 H+转运蛋白)和 TF(0)F(1)-ATP 合酶(一种热稳定的 ATP 合酶)的大肠杆菌细胞的外翻膜中制备了 ATP 光合囊泡。这些囊泡显示出光依赖性的 ATP 合成。此外,将 ATP 光合囊泡与 ATP 水解的己糖激酶偶联,可实现光依赖性的葡萄糖消耗。ATP 光合囊泡表明它们有潜力应用于光驱动的 ATP 再生生物工艺,用于各种 ATP 水解的生物产物。