Laboratório de Neuroproteção e Doença Metabólica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003 Porto Alegre, RS, Brazil.
Metab Brain Dis. 2011 Mar;26(1):61-7. doi: 10.1007/s11011-011-9232-3. Epub 2011 Feb 3.
In the present study we investigate the effect of homocysteine on glutamate uptake, Na+,K+-ATPase, enzymatic antioxidant defenses, as well as reactive species levels in hippocampus of rats. The influence of vitamin C, a classic antioxidant, on the effects elicited by homocysteine was also tested. Results showed that chronic hyperhomocysteinemia decreased glutamate uptake and the activities of Na+,K+-ATPase, catalase and superoxide dismutase in hippocampus of rats. Reactive species levels were increased by chronic homocysteine administration. Concomitant administration of vitamin C significantly prevented these alterations caused by homocysteine. According to our results, it seems possible to suggest that the reduction in glutamate uptake and Na+,K+-ATPase activity may be mediated by oxidative stress, since vitamin C prevented these effects. We suggest that the administration of antioxidants should be considered as an adjuvant therapy to specific diet in homocystinuria.
在本研究中，我们研究了同型半胱氨酸对谷氨酸摄取、Na+，K+-ATP 酶、酶抗氧化防御以及海马中活性物质水平的影响。还测试了维生素 C（一种经典的抗氧化剂）对同型半胱氨酸引起的这些影响的影响。结果表明，慢性高同型半胱氨酸血症降低了大鼠海马中的谷氨酸摄取和 Na+，K+-ATP 酶、过氧化氢酶和超氧化物歧化酶的活性。慢性同型半胱氨酸给药会增加活性物质的水平。维生素 C 的联合给药显著预防了同型半胱氨酸引起的这些变化。根据我们的结果，似乎可以提出，谷氨酸摄取和 Na+，K+-ATP 酶活性的降低可能是由氧化应激介导的，因为维生素 C 预防了这些作用。我们建议，在同型胱氨酸尿症中，应考虑将抗氧化剂的给药作为特定饮食的辅助治疗。
