[采用样本浓缩方法随后进行微型短串联重复序列基因分型对低拷贝数DNA进行法医分析]
[Forensic analysis of LCN DNA using sample concentration methods followed by miniSTR genotyping].
作者信息
Gu Li-Hua, Dong Yan, Zhang Chen, Xu Yan, Chen Rong-Hua, Hu Wei, Chen Lian-Kang, Zhou Huai-Gu
机构信息
Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
出版信息
Fa Yi Xue Za Zhi. 2010 Oct;26(5):361-3.
OBJECTIVE
To optimize low copy number (LCN) DNA analysis methods for forensic STR genotyping.
METHODS
Two groups of DNA sample, extracted using either Magnetic bead method or Chelex-100 methods, were previously amplified with a Identifiler PCR Amplification kit, but no genotype was detected. The DNA samples were concentrated using either a drying method or the Microcon-100 method, then amplified using an miniFiler PCR Amplification kit and genotyped.
RESULTS
Among the 127 DNA samples, 47 samples, previously extracted using the Magnetic bead method, were genotyped with 36% success rate. Eighty samples, previously extracted using the Chelex-100 method, were genotyped with 30% success rate.
CONCLUSION
The application of sample concentration methods and miniFiler kit can improve the success rate of LCN STR analysis.
目的
优化用于法医STR基因分型的低拷贝数（LCN）DNA分析方法。
方法
两组分别采用磁珠法或Chelex-100法提取的DNA样本，先前使用Identifiler PCR扩增试剂盒进行扩增，但未检测到基因型。DNA样本采用干燥法或Microcon-100法进行浓缩，然后使用miniFiler PCR扩增试剂盒进行扩增并进行基因分型。
结果
在127个DNA样本中，先前采用磁珠法提取的47个样本成功进行基因分型，成功率为36%。先前采用Chelex-100法提取的80个样本成功进行基因分型，成功率为30%。
结论
样本浓缩方法和miniFiler试剂盒的应用可提高LCN STR分析的成功率。
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