[快速标记22个STR基因座直接PCR扩增试剂盒的法医应用]

[Forensic application of expressmarker 22 STR loci direct PCR amplification kit].

作者信息

Zou Kai-Nan, Cao Yu, Xia Zi-Fang, Zheng Wei-Guo, Zhou Huai-Gu

机构信息

Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.

出版信息

Fa Yi Xue Za Zhi. 2012 Dec;28(6):448-50.

PMID:23484329

Abstract

OBJECTIVE

To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit.

METHODS

One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit.

RESULTS

97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler kit, Identifiler kit and PowerPlex 16 kit at the same STR loci.

CONCLUSION

Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results

摘要

目的

探讨Expressmarker 22个STR基因座直接PCR扩增试剂盒的应用价值。

方法

使用Expressmarker 22个STR基因座直接PCR扩增试剂盒对1948份样本（包括点样于FTA卡、滤纸上的样本及案件样本）进行检测。同时，分别使用Sinofiler试剂盒、Identifiler试剂盒和PowerPlex 16试剂盒对所有样本进行检测以作比较。比较这四种试剂盒在相同STR基因座处的基因型，以检测Expressmarker 22个STR基因座直接PCR扩增试剂盒的灵敏度和准确性。

结果

使用Expressmarker 22个STR基因座直接PCR扩增试剂盒97.79%的样本成功分型。使用Expressmarker 22个STR基因座直接PCR扩增试剂盒对相同样本在相同STR基因座处的基因型图谱与Sinofiler试剂盒、Identifiler试剂盒和PowerPlex 16试剂盒一致。