Yan Z Z, Wang W, Lu Z Y, Wu Y
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(3):161-4.
Circulating antigen in sera from acute, chronic and late stages of schistosomiasis patients was detected by direct dot-ELISA with monoclonal antibody 3D8A against schistosome gut-associated cathodic antigen linked with peroxidase, the positive rates being 90.6%, 83.2% and 30.7%, respectively. No positive reactions were found with sera from patients of clonorchiasis, malaria and non-parasitic diseases. The positive rate and the circulating antigen level in EPG greater than 100 group of patients were found to be higher than those in EPG less than 100 group. Circulating antigen became negative one year after praziquantel treatment in 84.0% of patients who showed negative fecal examination, while the other patients remained positive with decreasing titers. The results indicated that the circulating antigen in sera from schistosomiasis patients of various stages can be detected by dot-ELISA with monoclonal antibody 3D8A against circulating schistosome gut-associated cathodic antigen. The authors concluded that the circulating antigen level was correlated with the intensity of infection and the efficacy of treatment.
采用抗血吸虫肠相关阴极抗原的单克隆抗体3D8A与过氧化物酶交联，通过直接斑点酶联免疫吸附试验（dot-ELISA）检测血吸虫病患者急性、慢性和晚期血清中的循环抗原，阳性率分别为90.6%、83.2%和30.7%。华支睾吸虫病、疟疾和非寄生虫病患者的血清未出现阳性反应。EPG大于100组患者的阳性率和循环抗原水平高于EPG小于100组。在粪便检查呈阴性的患者中，84.0%的患者在吡喹酮治疗一年后循环抗原转阴，而其他患者仍为阳性，但滴度降低。结果表明，采用抗循环血吸虫肠相关阴极抗原的单克隆抗体3D8A的斑点酶联免疫吸附试验可检测各期血吸虫病患者血清中的循环抗原。作者得出结论，循环抗原水平与感染强度和治疗效果相关。
