[使用单克隆抗体的斑点酶联免疫吸附测定法检测血吸虫循环抗原]
[Dot-ELISA using monoclonal antibody for detecting schistosome circulating antigen].
作者信息
Zhou R, Miao D Q, Zhang S H, Zhang Y W, Zhu R S
机构信息
Institute of Antiparasitic Diseases, Sichuan Academy of Medical Sciences.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(4):267-9.
Monoclonal antibodies were produced by the fusion of splenic lymphocytes from BALB/c mice immunized with Schistosoma japonicum excretory/secretory antigen and the myeloma cell line SP2/0. The 1B2E7B8 McAb was proved to be specific against the gut antigen of adult worm in IFA. The McAb labelled with HRP was used in Dot-ELISA to detect schistosome circulating antigen. Schistosome circulating antigen was detected in 152 out of 188 proven cases of schistosomiasis, accounting for a positive rate of 80.9%. The positive rates for circulating antigen in cases with 1-24, 25-99 and greater than or equal to 100 EPG were 76.8%, 86.6% and 100% respectively. 10 out of 11 cases who had been checked 2 months after effective treatment became ELISA negative. No circulating antigen was detected in cases with other parasitosis nor in normal individuals. In addition, the McAb-Dot-ELISA showed good reproducibility. The results indicated that McAb-Dot-ELISA might be used for diagnosis of schistosomiasis and evaluation of cure.
用日本血吸虫排泄/分泌抗原免疫BALB/c小鼠，取其脾淋巴细胞与骨髓瘤细胞系SP2/0融合，制备单克隆抗体。间接荧光抗体试验（IFA）证明1B2E7B8单克隆抗体对成虫肠抗原具有特异性。用辣根过氧化物酶（HRP）标记的单克隆抗体用于斑点酶联免疫吸附测定（Dot-ELISA）检测血吸虫循环抗原。在188例确诊的血吸虫病病例中，152例检测到血吸虫循环抗原，阳性率为80.9%。每克粪便虫卵数（EPG）为1 - 24、25 - 99和大于或等于100的病例中循环抗原阳性率分别为76.8%、86.6%和100%。11例有效治疗2个月后复查的病例中，10例酶联免疫吸附测定（ELISA）转为阴性。其他寄生虫病患者及正常人均未检测到循环抗原。此外，单克隆抗体-斑点酶联免疫吸附测定（McAb-Dot-ELISA）具有良好的重复性。结果表明，McAb-Dot-ELISA可用于血吸虫病的诊断和疗效评价。
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