Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Chiba, Japan.
Dev Dyn. 2011 May;240(5):969-78. doi: 10.1002/dvdy.22552. Epub 2011 Feb 2.
During vertebrate limb development, various molecules are expressed in the presumptive limb field or the limb bud in a spatiotemporal-specific manner. The combination of these molecules regulates cellular properties that affect limb initiation and its morphogenesis, especially cartilage formation. Cell adhesiveness of the limb mesenchyme is a key factor in the regulation of cell distribution. Differential adhesiveness of mesenchymal cells is first observed between cells in the presumptive limb field and flank region, and the adhesiveness of the cells in the limb field is higher than that of cells in the flank region. In the limb bud, the adhesiveness of mesenchymal cells shows spatiotemporal difference, which reflects the positional identity of the cells. Position-dependent cell adhesiveness is also observed in blastema cells of the regenerating limb. Therefore, local changes in cell adhesiveness are observed during limb development and regeneration, suggesting significant roles for cell adhesiveness in limb morphogenesis.
在脊椎动物肢体发育过程中，各种分子以时空特异性的方式在假定的肢体区域或肢体芽中表达。这些分子的组合调节影响肢体起始及其形态发生的细胞特性，特别是软骨形成。肢体间质的细胞黏附性是调节细胞分布的关键因素。在假定的肢体区域和侧翼区域之间，间质细胞之间的黏附性首先表现出差异，并且肢体区域中的细胞的黏附性高于侧翼区域中的细胞的黏附性。在肢体芽中，间质细胞的黏附性表现出时空差异，反映了细胞的位置身份。在再生肢体的芽基细胞中也观察到位置依赖性细胞黏附性。因此，在肢体发育和再生过程中观察到细胞黏附性的局部变化，表明细胞黏附性在肢体形态发生中具有重要作用。
