Isaksson B, Hanberger H, Maller R, Nilsson L E, Nilsson M
Department of Infectious Diseases, University Hospital, Linköping, Sweden.
Scand J Infect Dis Suppl. 1990;74:129-32.
The in vitro postantibiotic effect (PAE) of amikacin was investigated using a bioluminescent assay of bacterial ATP. Two strains each of Escherichia coli, Pseudomonas aeruginosa and Serratia marcescens were exposed for one hour to different concentrations of amikacin. The aminoglycoside was removed by a 10(-3) dilution and regrowth of bacteria was followed at hourly intervals by monitoring bacterial ATP. The length of the PAE was concentration-dependent and was approximately four to six hours for the three strains at amikacin concentrations normally reached in serum during standard dosing. The PAE of amikacin in combination with 32 mg/l piperacillin on Ps. aeruginosa was also studied. These cultures were incubated with piperacillin for one hour. Thereafter different concentrations of amikacin 0.5-64 mg/l were added and the incubation then continued with the combinations for one more hour. The PAEs produced by the drugs in combination were longer than the sum of the individual effects of the drugs when they were used alone. Knowledge of synergistic PAE could have clinical implications for optimal dosing schedules during combination antimicrobial chemotherapy.
采用细菌ATP生物发光分析法研究了阿米卡星的体外抗生素后效应（PAE）。将大肠杆菌、铜绿假单胞菌和粘质沙雷氏菌各两株暴露于不同浓度的阿米卡星中1小时。通过10⁻³稀释去除氨基糖苷类药物，每隔一小时通过监测细菌ATP来跟踪细菌的再生长。PAE的时长呈浓度依赖性，在标准给药期间血清中通常达到的阿米卡星浓度下，这三种菌株的PAE约为4至6小时。还研究了阿米卡星与32mg/L哌拉西林联合使用对铜绿假单胞菌的PAE。这些培养物与哌拉西林孵育1小时。此后加入不同浓度的阿米卡星（0.5 - 64mg/L），然后将组合继续孵育1小时。联合使用药物产生的PAE比单独使用药物时各自效应的总和更长。协同PAE的知识可能对联合抗菌化疗期间的最佳给药方案具有临床意义。
