Isaksson B, Hanberger H, Maller R, Nilsson L E, Nilsson M
Department of Infectious Diseases, University Hospital, Linköping, Sweden.
J Antimicrob Chemother. 1991 Jul;28(1):25-34. doi: 10.1093/jac/28.1.25.
The post-antibiotic effect (PAE) of amikacin alone and in combination with ceftazidime, ceftriaxone and piperacillin was studied for two strains each of Pseudomonas aeruginosa and Serratia marcescens using a bioluminescent assay of bacterial ATP. Two models were used for combining beta-lactam antibiotics and amikacin: in one model the cultures were incubated with 32 mg/L of ceftazidime, 128 mg/L of ceftriaxone or 32 mg/L of piperacillin for 1 h. Different concentrations of amikacin (0.5-64 mg/L) were then added. Incubation of the combinations continued for one more hour. The antibiotics were eliminated by dilution. In the second model tested, one strain of S. marcescens was simultaneously exposed to amikacin and a beta-lactam antibiotic for 2 h. The PAEs produced by the drugs in combination were longer than the sum of the individual effects of the drugs when they were used alone. Results were equally good with both models. A synergic PAE was also found with amikacin concentrations close to the MIC in combination with low concentrations of ceftazidime, ceftriaxone and piperacillin.
使用细菌ATP生物发光测定法，对两株铜绿假单胞菌和粘质沙雷氏菌分别研究了阿米卡星单独使用以及与头孢他啶、头孢曲松和哌拉西林联合使用时的抗生素后效应（PAE）。使用了两种模型来联合β-内酰胺类抗生素和阿米卡星：在一种模型中，培养物与32 mg/L的头孢他啶、128 mg/L的头孢曲松或32 mg/L的哌拉西林孵育1小时。然后加入不同浓度的阿米卡星（0.5 - 64 mg/L）。联合孵育再持续1小时。通过稀释去除抗生素。在测试的第二种模型中，一株粘质沙雷氏菌同时暴露于阿米卡星和一种β-内酰胺类抗生素2小时。联合用药产生的PAE比单独使用药物时各自效应的总和更长。两种模型的结果同样良好。还发现，在与低浓度的头孢他啶、头孢曲松和哌拉西林联合使用时，接近最低抑菌浓度（MIC）的阿米卡星浓度也会产生协同PAE。
