[富集突触体、突触小泡和突触质膜的分离]

[Separation of enriched synaptosomes, synaptic vesicles and synaptic plasma membranes].

作者信息

Franke L, Uebelhack R

出版信息

Acta Biol Med Ger. 1978;37(2):205-13.

Abstract

A rapid and simple method is described for separation of intact synaptosomes, synaptic plasma membranes and vesicles. Two synaptosome fractions were obtained by modified differential centrifugation. The rate zonal zentrifugation in a linear sucrose gradient (very low density) is suitable to obtain fractions highly enriched in synaptic plasma membranes and vesicles. Examination of the prepared fractions was done by enzyme marker activities and electron microscopy

摘要

描述了一种快速简便的方法用于分离完整的突触体、突触质膜和囊泡。通过改良的差速离心获得了两个突触体组分。在线性蔗糖梯度（极低密度）中进行速率区带离心，适合获得高度富集突触质膜和囊泡的组分。通过酶标记活性和电子显微镜对制备的组分进行检测。