Mylonakis Mathios E, Borjesson Dori L, Leontides Leonidas, Siarkou Victoria I, Theodorou Konstantina, Koutinas Alexander F
Companion Animal Clinic, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Vet Clin Pathol. 2011 Mar;40(1):78-83. doi: 10.1111/j.1939-165X.2011.00293.x. Epub 2011 Feb 3.
Recognition of different cytologic patterns in lymph nodes (LNs) from dogs with canine monocytic ehrlichiosis (CME) and noninfectious causes of lymphoid reactivity may have diagnostic utility.
The aims of the present study were to compare cytologic patterns in LNs of dogs with different phases of CME, to investigate the association of cytologic pattern and presence of Ehrlichia spp. morulae, and to compare patterns of lymphoid reactivity between dogs with CME and those with noninfectious causes of lymphoid hyperplasia.
Cytologic preparations of LNs from 35 dogs with nonmyelosuppressive CME (group A), 16 dogs with myelosuppressive CME (group B), 26 dogs with noninfectious diseases (group C), and 15 healthy dogs (group D) were evaluated. Percentages of lymphocyte types, plasma cells, macrophages, neutrophils, and eosinophils were determined. Samples from dogs in groups A and B were evaluated for the presence of morulae.
Cytologic abnormalities in LNs were recorded in 54% of dogs in group A, 88% in group B, 39% in group C, and 0% in group D and were more frequent (P=.02) in dogs with myelosuppressive CME than those with nonmyelosuppressive CME. Plasma cell hyperplasia was more frequent in CME than in noninfectious diseases (P=.03). An association between the presence of cytologic abnormalities and morulae in group A dogs was not found.
Dogs with myelosuppressive CME have more lymphoid cytologic abnormalities than dogs with nonmyelosuppressive CME. LN plasmacytosis is the major pattern of lymphadenopathy in dogs with CME and is found more frequently in dogs with CME than in dogs with noninfectious causes of lymphadenopathy.
识别患有犬单核细胞埃立克体病（CME）的犬只淋巴结（LN）中的不同细胞学模式以及非感染性淋巴反应原因可能具有诊断价值。
本研究的目的是比较处于CME不同阶段的犬只淋巴结中的细胞学模式，研究细胞学模式与埃立克体属桑葚体存在之间的关联，并比较患有CME的犬只与患有非感染性淋巴增生原因的犬只之间的淋巴反应模式。
对35只患有非骨髓抑制性CME的犬只（A组）、16只患有骨髓抑制性CME的犬只（B组）、26只患有非感染性疾病的犬只（C组）和15只健康犬只（D组）的淋巴结进行细胞学制片评估。确定淋巴细胞类型、浆细胞、巨噬细胞、中性粒细胞和嗜酸性粒细胞的百分比。对A组和B组犬只的样本进行桑葚体检测。
A组54%的犬只、B组88%的犬只、C组39%的犬只和D组0%的犬只出现淋巴结细胞学异常，骨髓抑制性CME犬只的异常情况比非骨髓抑制性CME犬只更常见（P = 0.02）。CME中浆细胞增生比非感染性疾病更常见（P = 0.03）。未发现A组犬只细胞学异常与桑葚体存在之间的关联。
患有骨髓抑制性CME的犬类比患有非骨髓抑制性CME的犬类有更多的淋巴细胞细胞学异常。淋巴结浆细胞增多是患有CME犬只淋巴结病的主要模式，且在患有CME的犬只中比在患有非感染性淋巴结病原因的犬只中更常见。
