Amrita Center for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Viswa Vidyapeetham, Kochi 682 041, India.
Int J Biol Macromol. 2011 May 1;48(4):571-6. doi: 10.1016/j.ijbiomac.2011.01.020. Epub 2011 Feb 1.
Chitosan/poly(caprolactone) (CS/PCL) nanofibrous scaffold was prepared by a single step electrospinning technique. The presence of CS in CS/PCL scaffold aided a significant improvement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle, which thereby enhanced bioactivity and protein adsorption on the scaffold. The cyto-compatibility of the CS/PCL scaffold was examined using human osteoscarcoma cells (MG63) and found to be non toxic. Moreover, CS/PCL scaffold was found to support the attachment and proliferation of various cell lines such as mouse embryo fibroblasts (NIH3T3), murine aneuploid fibro sarcoma (L929), and MG63 cells. Cell attachment and proliferation was further confirmed by nuclear staining using 4',6-diamidino-2-phenylindole (DAPI). All these results indicate that CS/PCL nanofibrous scaffold would be an excellent system for bone and skin tissue engineering.
壳聚糖/聚己内酯（CS/PCL）纳米纤维支架是通过一步电纺技术制备的。CS 的存在有助于 CS/PCL 支架的亲水性显著提高，这一点通过接触角的降低得到了证实，从而提高了支架的生物活性和蛋白质吸附能力。通过使用人骨肉瘤细胞（MG63）对 CS/PCL 支架的细胞相容性进行了检测，结果表明其无毒。此外，CS/PCL 支架还支持各种细胞系（如小鼠胚胎成纤维细胞（NIH3T3）、鼠染色体非整倍体纤维肉瘤（L929）和 MG63 细胞）的附着和增殖。通过使用 4'，6-二脒基-2-苯基吲哚（DAPI）进行核染色进一步证实了细胞附着和增殖。所有这些结果表明，CS/PCL 纳米纤维支架将是骨和皮肤组织工程的优秀系统。
