Cui Zhixiang, Lin Luyin, Si Junhui, Luo Yufei, Wang Qianting, Lin Yongnan, Wang Xiaofeng, Chen Wenzhe
a School of Materials Science and Engineering, Fujian University of Technology , Fuzhou , China.
b Fujian Provincial Key Laboratory of Advanced Materials Processing and Application , Fuzhou , China.
J Biomater Sci Polym Ed. 2017 Jun;28(9):826-845. doi: 10.1080/09205063.2017.1303867. Epub 2017 Mar 14.
As one of the stimulators on bone formation, osteogenic growth peptide (OGP) improves both proliferation and differentiation of the bone cells in vitro and in vivo. The aim of this work was the preparation of three dimensional porous poly(ε-caprolactone) (PCL) scaffold with high porosity, well interpore connectivity, and then its surface was modified by using chitosan (CS)/OGP coating for application in bone regeneration. In present study, the properties of porous PCL and CS/OGP coated PCL scaffold, including the microstructure, water absorption, porosity, hydrophilicity, mechanical properties, and biocompatibility in vitro were investigated. Results showed that the PCL and CS/OGP-PCL scaffold with an interconnected network structure have a porosity of more than 91.5, 80.8%, respectively. The CS/OGP-PCL scaffold exhibited better hydrophilicity and mechanical properties than that of uncoated PCL scaffold. Moreover, the results of cell culture test showed that CS/OGP coating could stimulate the proliferation and growth of osteoblast cells on CS/OGP-PCL scaffold. These finding suggested that the surface modification could be a effective method on enhancing cell adhesion to synthetic polymer-based scaffolds in tissue engineering application and the developed porous CS/OGP-PCL scaffold should be considered as alternative biomaterials for bone regeneration.
作为骨形成的刺激因子之一，成骨生长肽（OGP）在体内外均可促进骨细胞的增殖和分化。本研究旨在制备具有高孔隙率、良好孔间连通性的三维多孔聚己内酯（PCL）支架，并通过壳聚糖（CS）/OGP涂层对其表面进行改性，以应用于骨再生。在本研究中，对多孔PCL和CS/OGP涂层PCL支架的性能进行了研究，包括微观结构、吸水性、孔隙率、亲水性、力学性能和体外生物相容性。结果表明，具有相互连通网络结构的PCL和CS/OGP-PCL支架的孔隙率分别超过91.5%和80.8%。CS/OGP-PCL支架比未涂层的PCL支架表现出更好的亲水性和力学性能。此外，细胞培养测试结果表明，CS/OGP涂层可促进成骨细胞在CS/OGP-PCL支架上的增殖和生长。这些发现表明，表面改性可能是在组织工程应用中增强细胞对合成聚合物基支架粘附的有效方法，所制备的多孔CS/OGP-PCL支架应被视为骨再生的替代生物材料。
