Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de València, Postal Code 22085, 46071 València, Spain.
J Biotechnol. 2011 Mar 20;152(3):93-5. doi: 10.1016/j.jbiotec.2011.01.005. Epub 2011 Feb 1.
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5 V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
在这项研究中，我们展示了直接对一种转基因酵母菌株中表达的水母发光钙激活蛋白——水母发光蛋白进行外部电刺激的应用。酵母培养物通过与标准电源连接的两个电极进行电刺激。即使是低强度的（1.5 V）电脉冲也能引发快速的光峰，并且在周期性施加电脉冲后可以获得连续的光脉冲，这表明该系统在短暂的折射时间后可以重新发挥作用。这些结果开辟了一个新的场景，即在合成生物学和控制论之间的非常交界地带，其中复杂的细胞行为可能受到电控制。
