Department of Urology, Huashan Hospital, Fudan University, Shanghai, China.
Urol Oncol. 2013 Feb;31(2):241-6. doi: 10.1016/j.urolonc.2010.12.001. Epub 2011 Feb 2.
Angiogenesis is a pivotal process on which solid tumor growth is substantially dependent. Pigment epithelium-derived factor (PEDF) is the most potent natural anti-angiogenic factor, which has seldom been studied in bladder tumor, and whose functioning pathway remains unclear. We have thus investigated PEDF expression in relation to tumor necrosis factor-α (TNF-α) and microvessel density (MVD) with immunohistochemistry.
Antibodies of PEDF and TNF-α were examined by Western blotting before immunohistochemistry. Sixty-four urothelial tumor sections and 23 normal controls were stained and expression of PEDF, TNF-α, and MVD were studied.
Decreased PEDF expression and increased TNF-α expression was noticed in tumorous tissue compared with healthy urothelium. Lower PEDF expression was related to higher tumor grade but stage. Increased TNF-α expression was noticed in recurrent, larger tumors as well as in tumors with progression in grade and stage. Expression of PEDF and TNF-α was correlated in bladder tumor. PEDF or TNF-α was correlated with MVD negatively or positively, respectively, in cancerous tissue and tumorous grouping without correlation in papillary urothelial neoplasm of low malignant potential.
Expressional change of PEDF and TNF-α is in relation to angiogenesis of bladder tumor, especially in bladder cancer development.
血管生成是实体肿瘤生长的关键过程。色素上皮衍生因子（PEDF）是最有效的天然抗血管生成因子，在膀胱肿瘤中很少被研究，其作用途径尚不清楚。因此，我们通过免疫组织化学研究了 PEDF 表达与肿瘤坏死因子-α（TNF-α）和微血管密度（MVD）的关系。
在免疫组织化学染色之前，用 Western blot 检测 PEDF 和 TNF-α抗体。对 64 例尿路上皮肿瘤切片和 23 例正常对照进行染色，并研究 PEDF、TNF-α和 MVD 的表达。
与健康尿路上皮相比，肿瘤组织中 PEDF 表达降低，TNF-α表达增加。较低的 PEDF 表达与较高的肿瘤分级但分期相关。在复发性、较大的肿瘤以及在分级和分期进展的肿瘤中，TNF-α的表达增加。在膀胱癌中，PEDF 和 TNF-α的表达呈正相关。在癌组织中，PEDF 或 TNF-α与 MVD 呈负相关或正相关，而在低度恶性潜能的乳头状尿路上皮肿瘤中无相关性。
PEDF 和 TNF-α的表达变化与膀胱肿瘤的血管生成有关，特别是在膀胱癌的发展中。
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