Kreuchwig Annika, Kleinau Gunnar, Kreuchwig Franziska, Worth Catherine L, Krause Gerd
Leibniz-Institut für Molekulare Pharmakologie, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
Mol Endocrinol. 2011 Apr;25(4):707-12. doi: 10.1210/me.2010-0510. Epub 2011 Feb 3.
The SSFA-GPHR (Sequence-Structure-Function-Analysis of Glycoprotein Hormone Receptors) database provides a comprehensive set of mutation data for the glycoprotein hormone receptors (covering the lutropin, the FSH, and the TSH receptors). Moreover, it provides a platform for comparison and investigation of these homologous receptors and helps in understanding protein malfunctions associated with several diseases. Besides extending the data set (> 1100 mutations), the database has been completely redesigned and several novel features and analysis tools have been added to the web site. These tools allow the focused extraction of semiquantitative mutant data from the GPHR subtypes and different experimental approaches. Functional and structural data of the GPHRs are now linked interactively at the web interface, and new tools for data visualization (on three-dimensional protein structures) are provided. The interpretation of functional findings is supported by receptor morphings simulating intramolecular changes during the activation process, which thus help to trace the potential function of each amino acid and provide clues to the local structural environment, including potentially relocated spatial counterpart residues. Furthermore, double and triple mutations are newly included to allow the analysis of their functional effects related to their spatial interrelationship in structures or homology models. A new important feature is the search option and data visualization by interactive and user-defined snake-plots. These new tools allow fast and easy searches for specific functional data and thereby give deeper insights in the mechanisms of hormone binding, signal transduction, and signaling regulation. The web application "Sequence-Structure-Function-Analysis of GPHRs" is accessible on the internet at http://www.ssfa-gphr.de/.
糖蛋白激素受体序列-结构-功能分析（SSFA-GPHR）数据库提供了一套全面的糖蛋白激素受体突变数据（涵盖促黄体激素、促卵泡激素和促甲状腺激素受体）。此外，它还为这些同源受体的比较和研究提供了一个平台，有助于理解与多种疾病相关的蛋白质功能异常。除了扩展数据集（超过1100个突变）外，该数据库已进行了全面重新设计，并在网站上添加了几个新的功能和分析工具。这些工具允许从GPHR亚型和不同实验方法中重点提取半定量突变数据。GPHR的功能和结构数据现在在网络界面上进行交互式链接，并提供了新的数据可视化工具（用于三维蛋白质结构）。受体变形模拟激活过程中的分子内变化，支持对功能发现的解释，从而有助于追踪每个氨基酸的潜在功能，并为局部结构环境提供线索，包括潜在重新定位的空间对应残基。此外，新纳入了双突变和三突变，以分析它们与结构或同源模型中空间相互关系相关的功能效应。一个新的重要功能是通过交互式和用户定义的蛇形图进行搜索和数据可视化。这些新工具允许快速轻松地搜索特定功能数据，从而更深入地了解激素结合、信号转导和信号调节机制。“糖蛋白激素受体序列-结构-功能分析”网络应用程序可通过互联网访问，网址为http://www.ssfa-gphr.de/ 。
