Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, PR China.
Intervirology. 2012;55(1):68-72. doi: 10.1159/000323524. Epub 2011 Feb 5.
BACKGROUND/AIMS: The present study was launched to systematically characterize the molecular genotypes and quantitative serological biomarkers during the natural course of chronic hepatitis B (CHB) infection in a patient population from Chongqing, China.
A total of 140 patients were enrolled and grouped according to the natural phases of HBV infection: HBeAg (+) immune tolerance, immune clearance, HBeAg (-) low replicative and reactivation phase. Nested PCR was used for HBV molecular genotyping and chemiluminescent microparticle immunoassay for quantitative serological detection.
Subgenotypes B2 and C2 were found to be most prevalent with genotype B patients showing more potent viral activity. HBsAg level was found to be significantly correlated with HBV DNA, HBeAg, age, ALT and AST levels during some phases of CHB.
These results indicated that molecular genotyping and quantitative serological detection might be helpful for earlier prediction of the long-term disease outcomes.
背景/目的：本研究旨在系统地描述中国重庆患者人群慢性乙型肝炎（CHB）感染自然病程中的分子基因型和定量血清学标志物。
共纳入 140 例患者，并根据 HBV 感染的自然阶段进行分组：HBeAg（+）免疫耐受、免疫清除、HBeAg（-）低复制和再激活期。巢式 PCR 用于 HBV 分子基因分型，化学发光微粒子免疫测定法用于定量血清学检测。
发现亚基因型 B2 和 C2 最为常见，基因型 B 患者的病毒活性更强。在 CHB 的某些阶段，HBsAg 水平与 HBV DNA、HBeAg、年龄、ALT 和 AST 水平显著相关。
这些结果表明，分子基因分型和定量血清学检测可能有助于更早预测长期疾病结局。
