Department of Periodontics, Seoul National University Dental Hospital, 101 Daehakno, Jongno-gu, Seoul, 03080, Korea.
Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, 101 Daehakno, Jongno-gu, Seoul, 03080, Korea.
Clin Oral Investig. 2021 Nov;25(11):6159-6170. doi: 10.1007/s00784-021-03915-7. Epub 2021 May 3.
We previously suggested an ovariectomy (OVX)-induced osteoporotic rat model showing an impaired alveolar bone defect healing. This study aimed to evaluate and compare the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and recombinant human platelet-derived growth factor-BB (rhPDGF-BB) on alveolar bone defect healing in OVX-induced osteoporotic rats.
A total of forty-one female rats were divided into four groups: a collagen group (n=10), a PDGF-BB group (n=11), a BMP-2 group (n=10), and a control group (n=10). Four months after OVX, alveolar bone drill-hole defects were created and grafted with collagen gel, rhPDGF-BB/collagen gel, or rhBMP-2/collagen gel. The defects in the control group were not grafted with any material. Defect healing was evaluated by histological, histomorphometric, and microcomputed tomographic (micro-CT) analyses at 2 and 4 weeks.
According to the micro-CT analysis, the BMP-2 group exhibited the greatest bone volume fraction among all groups, while the PDGF-BB group did not show significant differences compared with the collagen group. The histomorphometric analysis showed a significantly larger amount of new bone area in the BMP-2 group than in the control and collagen groups at 4 weeks; however, the PDGF-BB group did not reach significant superiority compared with the other groups.
Alveolar bone regeneration was significantly enhanced by the local use of rhBMP-2/collagen gel compared with the use of rhPDGF-BB/collagen gel in OVX-induced osteoporotic rats.
A treatment modality using rhBMP-2 may be a promising approach to promote alveolar bone regeneration in patients suffering from postmenopausal osteoporosis.
我们之前提出了一个去卵巢（OVX）诱导的骨质疏松症大鼠模型，该模型显示牙槽骨缺损愈合受损。本研究旨在评估和比较重组人骨形态发生蛋白-2（rhBMP-2）和重组人血小板衍生生长因子-BB（rhPDGF-BB）对 OVX 诱导的骨质疏松症大鼠牙槽骨缺损愈合的影响。
共 41 只雌性大鼠分为四组：胶原组（n=10）、PDGF-BB 组（n=11）、BMP-2 组（n=10）和对照组（n=10）。OVX 后 4 个月，在牙槽骨钻孔缺陷处植入胶原凝胶、rhPDGF-BB/胶原凝胶或 rhBMP-2/胶原凝胶。对照组的缺陷未用任何材料进行移植。在 2 周和 4 周时通过组织学、组织形态计量学和微计算机断层扫描（micro-CT）分析评估缺损愈合情况。
根据 micro-CT 分析，BMP-2 组在所有组中表现出最大的骨体积分数，而 PDGF-BB 组与胶原组相比没有显著差异。组织形态计量学分析显示，在 4 周时，BMP-2 组的新骨面积明显大于对照组和胶原组；然而，PDGF-BB 组与其他组相比没有达到显著优势。
与 rhPDGF-BB/胶原凝胶相比，rhBMP-2/胶原凝胶局部应用可显著增强 OVX 诱导的骨质疏松症大鼠牙槽骨再生。
使用 rhBMP-2 的治疗方法可能是促进绝经后骨质疏松症患者牙槽骨再生的一种有前途的方法。
