Department of Chemistry, Queen's University, 90 Bader Lane, Kingston, Ontario, Canada K7P 3E5.
Biomacromolecules. 2011 Mar 14;12(3):813-23. doi: 10.1021/bm101432e. Epub 2011 Feb 4.
Water-dispersible superparamagnetic polymer/γ-Fe(2)O(3) composite microspheres adorned with two types of surface polymer chains are prepared and characterized. To prepare these spheres, we first synthesize uniform γ-Fe(2)O(3) nanoparticles that are covered by poly(2-cinnamoyloxyethyl methacrylate)-block-poly(acrylic acid) (PCEMA-b-PAA). These nanoparticles are then mixed with a PCEMA homopolymer in CHCl(3) to form an oil phase. The oil phase is dispersed into water under vigorous stirring with the help of two diblock copolymer surfactants, PGMA-b-PCEMA and PSGMA-b-PCEMA. Here PGMA and PSGMA denote poly(glyceryl monomethacrylate) and succinated PGMA, respectively. Solid microspheres with cores composed of PCEMA and PCEMA-b-PAA-covered γ-Fe(2)O(3) nanoparticles are obtained after CHCl(3) evaporation and PCEMA photo-cross-linking. Under certain conditions, the coronal PGMA and PSGMA chains become segregated, thus producing surface bumps, ridges, and valleys. The PSGMA chains preferentially cover the protruding regions. The PSGMA carboxyl groups are used to immobilize bovine serum albumin (BSA). The immobilized BSA retains its activity and binds with anti-BSA. These spheres should be useful in immunoassays.
水散性超顺磁聚合物/γ-Fe(2)O(3)复合微球，表面修饰有两种类型的聚合物链，对其进行了制备和表征。为了制备这些微球，我们首先合成了均匀的 γ-Fe(2)O(3)纳米粒子，其表面覆盖有聚(2-肉桂酰氧基乙基甲基丙烯酸酯)-嵌段-聚(丙烯酸)（PCEMA-b-PAA）。然后，将这些纳米粒子与 PCEMA 均聚物在 CHCl(3)中混合，形成油相。在两种两亲性嵌段共聚物表面活性剂 PGMA-b-PCEMA 和 PSGMA-b-PCEMA 的帮助下，将油相强力搅拌分散到水中。这里 PGMA 和 PSGMA 分别表示聚（甘油单甲基丙烯酸酯）和琥珀酰化 PGMA。CHCl(3)蒸发和 PCEMA 光交联后，得到了由 PCEMA 和 PCEMA-b-PAA 覆盖的 γ-Fe(2)O(3)纳米粒子组成的核的实心微球。在某些条件下，冠状 PGMA 和 PSGMA 链会发生分离，从而产生表面凸起、脊和谷。PSGMA 链优先覆盖突出区域。PSGMA 羧基用于固定牛血清白蛋白（BSA）。固定的 BSA 保留其活性并与抗-BSA 结合。这些微球在免疫分析中应该是有用的。
