Oh-Hara T, Sakagami H, Kawazoe Y, Kaiya T, Komatsu N, Ohsawa N, Fujimaki M, Tanuma S, Konno K
Faculty of Pharmaceutical Sciences, Nagoya City University, Japan.
In Vivo. 1990 Jan-Feb;4(1):7-12.
Pine cones of Pinus parviflora Sieb., et Zucc, were extracted successively with 5% NaHCO3, 3% NH4OH, 1% NaOH and 4% NaOH, and the extracts were tested for ability to induce antimicrobial activity in mice infected with Staphylococcal aureus. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Candida albicans, or Salmonella enteritidis. These alkaline extracts were potent against the first 5 of these strains of microorganisms, and the activity was recovered mostly from their acid-precipitates at pH 5. After further fractionation on a Sephadex LH-60 column, the highest molecular weight fraction was most potent. Chemical structures in these bioactive fractions were assumed to be lignin-related structures, based on spectral data from UV, NMR and IR spectroscopy.
对赤松的松果依次用5%碳酸氢钠、3%氢氧化铵、1%氢氧化钠和4%氢氧化钠进行提取，然后对提取物进行测试，以检测其对感染金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌、白色念珠菌或肠炎沙门氏菌的小鼠诱导抗菌活性的能力。这些碱性提取物对上述前5种微生物菌株具有强效作用，且活性大多从其pH值为5的酸沉淀物中恢复。在Sephadex LH - 60柱上进一步分级分离后，分子量最高的级分活性最强。根据紫外光谱、核磁共振光谱和红外光谱的光谱数据，推测这些生物活性级分中的化学结构为木质素相关结构。
