Napier J A, Höglund A S, Plant A L, Gray J C
Department of Plant Sciences, University of Cambridge, UK.
Plant Mol Biol. 1992 Nov;20(4):737-41. doi: 10.1007/BF00046458.
A cDNA clone encoding the complete precursor of the gamma subunit of the pea chloroplast ATP synthase has been isolated from a pea leaf cDNA library in lambda gt 11 following detection with antibodies to the purified gamma subunit. The cDNA insert of 1.4 kbp is smaller than transcripts of about 1.6 kb detected by northern hybridisation of RNA from both light- and darkgrown pea leaves. The cDNA encodes a polypeptide of 376 amino acid residues, of which 52 residues constitute an N-terminal presequence and 324 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a protein of 42 kDa, which was imported by isolated pea chloroplasts and processed to the mature 36 kDa subunit.
在用纯化的γ亚基抗体进行检测后，从λgt 11载体构建的豌豆叶片cDNA文库中分离出了一个编码豌豆叶绿体ATP合酶γ亚基完整前体的cDNA克隆。1.4 kbp的cDNA插入片段比从光生长和暗生长的豌豆叶片RNA经Northern杂交检测到的约1.6 kb的转录本小。该cDNA编码一个由376个氨基酸残基组成的多肽，其中52个残基构成N端前导序列，324个残基组成成熟蛋白。该cDNA在体外转录和翻译产生了一个42 kDa的蛋白质，该蛋白质被分离的豌豆叶绿体导入并加工成成熟的36 kDa亚基。
