Department of Veterinary Clinical Sciences, University of Teramo, Teramo, Italy.
Theriogenology. 2011 Apr 15;75(7):1319-26. doi: 10.1016/j.theriogenology.2010.12.003. Epub 2011 Feb 4.
In this study, the effect of dietary supplementation of organic selenium, vitamin E, and zinc on raw semen characteristics was evaluated. Ten stallions with normal fertility were divided into two groups: a control group (CG), in which standard diet was provided, and a treated group (TG), in which the standard diet was supplemented with 1500 mg of α-tocopherol acetate, 360 mg of zinc, and 2.5 mg of organic selenium on a daily basis. Semen parameters on fresh semen were evaluated three times in all stallions before antioxidant supplementation (T0) and 30 (T1), 60 (T2), and 90 (T3) d after supplementation. Dietary supplementation with experimental antioxidants resulted in a significant increase in average path velocity (121.9 ± 3.1 μm/sec in TG vs 118.9 ± 4.3 μm/sec in CG), straightness (86.2 ± 2.4 % vs 82.6 ± 3.9 % in TG and CG respectively), viability (75.6 ± 10.2 % in TG vs 72.3 ± 6.9 % in CG) and total seminal plasma antioxidants levels (2.7 ± 0.5 mmol/l vs 1.9 ± 0.4 mmol/l in TG and CG respectively) while progressive motility 69.7 ± 11 % vs 62.2 ± 9.3 % in TG and CG stallions respectively) and abnormal sperm morphology (8.2±1.5 % in TG vs 14.4±4 % in CG) significantly improved in treated stallions after 60 d of supplementation. In contrast with previously reported in other species, a negative effect of antioxidant supplementation on semen concentration was recorded in the TG. A positive correlation between progressive motility and total antioxidants in seminal plasma in both treated and control stallions suggested that motility is affected by oxidative-antioxidative status, and that dietary antioxidant supplementation could increase the ability of spermatozoa to contrast reactive oxygen species or the ability of seminal plasma to reduce the oxidative stress. The improvement of semen parameters after antioxidant supplementation was not linear, and after 30 d (or 60 d for some parameters), a further increase was not noted. This evidence suggested that in our standard conditions, dietary intake of these antioxidants could be slightly under the dietary requirement and further evaluation of the actual nutrition requirements of organic selenium, zinc, and vitamin E in the stallion are needed.
在这项研究中，评估了日粮补充有机硒、维生素 E 和锌对鲜精特性的影响。将 10 匹具有正常生育力的种马分为两组：对照组（CG），提供标准日粮；处理组（TG），在标准日粮中每天补充 1500mgα-生育酚醋酸酯、360mg 锌和 2.5mg 有机硒。在所有种马进行抗氧化剂补充前（T0）和补充后 30（T1）、60（T2）和 90（T3）天，三次评估新鲜精液的精液参数。日粮补充试验抗氧化剂可显著提高平均路径速度（TG 为 121.9±3.1μm/sec，CG 为 118.9±4.3μm/sec）、直线性（TG 为 86.2±2.4%，CG 为 82.6±3.9%）、活力（TG 为 75.6±10.2%，CG 为 72.3±6.9%）和总精液血浆抗氧化剂水平（TG 为 2.7±0.5mmol/l，CG 为 1.9±0.4mmol/l），而运动性（TG 为 69.7±11%，CG 为 62.2±9.3%）和异常精子形态（TG 为 8.2±1.5%，CG 为 14.4±4%）在 TG 处理种马补充 60 天后显著改善。与其他物种的先前报道相反，TG 中抗氧化剂补充对精液浓度有负面影响。在处理和对照种马中，前向运动与精液血浆总抗氧化剂之间呈正相关，这表明运动性受氧化还原状态的影响，日粮抗氧化剂补充可以提高精子抵抗活性氧的能力或精液降低氧化应激的能力。抗氧化剂补充后精液参数的改善不是线性的，在 30 天后（或某些参数 60 天后），没有注意到进一步的增加。这一证据表明，在我们的标准条件下，这些抗氧化剂的日粮摄入可能略低于日粮需求，需要进一步评估种马对有机硒、锌和维生素 E 的实际营养需求。
