马精液总活力和膜完整性测量之间的一致性。
Agreement between measures of total motility and membrane integrity in stallion sperm.
机构信息
Department of Large Animal Clinical Sciences, Texas A&M University, College Station, TX, USA.
出版信息
Theriogenology. 2011 May;75(8):1499-505. doi: 10.1016/j.theriogenology.2010.12.011. Epub 2011 Feb 4.
Increasing seminal plasma concentrations in extended stallion semen were utilized to model decreasing sperm motility over time. Level of agreement was determined between flow cytometric measurement of sperm membrane integrity, using a combination of SYBR-14 and propidium iodide, and computer-assisted analysis of sperm motility. Values for total sperm motility (TMOT;%) and membrane integrity (SMI;%) were similar (∼80%) at Time 0 within all sperm treatments. However, TMOT was lower than SMI after 24 and 48 h of storage in treatments with >20% seminal plasma. At Time 0, agreement (bias and absolute difference) between TMOT and SMI was high (-0.7 and 5.6%, respectively), but decreased after 24 (10.8 and 15.1%, respectively) and 48 h (23.0 and 23.8%, respectively) of cooled storage as motility declined more rapidly than SMI. We concluded that TMOT and SMI measured separate aspects of sperm quality.
在延长种马精液的静止时间过程中，增加精浆浓度被用于模拟精子活力随时间的下降。使用 SYBR-14 和碘化丙啶的组合，通过流式细胞术测量精子膜完整性，并与计算机辅助的精子运动分析进行比较，以确定两者之间的一致性。在所有精液处理中，0 时间的总精子活力（TMOT；%）和膜完整性（SMI；%）相似（约 80%）。然而，在静止时间超过 20%的精液处理中，24 小时和 48 小时的储存后，TMOT 低于 SMI。在 0 时间，TMOT 和 SMI 之间的一致性（偏差和绝对差异）较高（分别为-0.7%和 5.6%），但在 24 小时（分别为 10.8%和 15.1%）和 48 小时（分别为 23.0%和 23.8%）的冷却储存后，随着精子活力的下降速度快于 SMI，一致性降低。我们得出结论，TMOT 和 SMI 测量了精子质量的不同方面。
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