Graduate School of Environmental Studies, Tohoku University, Aoba, Sendai, Japan.
Colloids Surf B Biointerfaces. 2011 May 1;84(1):181-6. doi: 10.1016/j.colsurfb.2010.12.033. Epub 2011 Jan 19.
We have prepared several types of polymers derived from 2-methacryloyloxyethyl phosphorylcholine (MPC) to evaluate whether polymers of MPC work as cell membrane mimic or not. We firstly applied capturing test of target proteins of 4-carboxybenzenesulfonamide (Sul) or ibuprofen (Ibu) as a probe. As the results, the rather hydrophilic polymers based on MPC were able to suppress non-specific binding proteins as expected. Additionally, some of the MPC based polymeric surface was able to capture greater amount of carbonic anhydrase II than those of other polymers, when Sul was utilized as probe. In contrary, all the polymers having PC groups and Ibu probe were unable to capture Cyclooxygenase-1 (COX-1), its target protein. These results suggested that the position of PC groups realized hydrophilic polymer surface, while MPC based polymer was not able to supply the suitable environment for COX-1 to interact with Ibu.
我们已经制备了几种源自 2-（甲基丙烯酰氧）乙基磷酸胆碱（MPC）的聚合物，以评估 MPC 聚合物是否可以模拟细胞膜。我们首先应用 4-羧基苯磺酰胺（Sul）或布洛芬（Ibu）作为探针的靶蛋白捕获试验。结果表明，基于 MPC 的相当亲水的聚合物能够如预期的那样抑制非特异性结合蛋白。此外，当 Sul 用作探针时，一些基于 MPC 的聚合表面能够捕获比其他聚合物更多的碳酸酐酶 II。相反，所有具有 PC 基团和 Ibu 探针的聚合物都无法捕获环氧化酶-1（COX-1），即其靶蛋白。这些结果表明，PC 基团的位置实现了亲水聚合物表面，而基于 MPC 的聚合物则不能为 COX-1 与 Ibu 相互作用提供合适的环境。
