Laboratory of Biochemical Engineering, College of Chemistry and Food Engineering, Federal University of Rio Grande (FURG), P.O. Box 474, 96201-900, Rio Grande-RS, Brazil.
Bioresour Technol. 2011 Apr;102(7):4897-900. doi: 10.1016/j.biortech.2010.12.011. Epub 2010 Dec 7.
We evaluated the kinetic culture characteristics of the microalgae Cyanobium sp. grown in vertical tubular photobioreactor in semicontinuous mode. Cultivation was carried out in vertical tubular photobioreactor for 2 L, in 57 d, at 30 °C, 3200 Lux, and 12 h light/dark photoperiod. The maximum specific growth rate was found as 0.127 d(-1), when the culture had blend concentration of 1.0 g L(-1), renewal rate of 50%, and sodium bicarbonate concentration of 1.0 g L(-1). The maximum values of productivity (0.071 g L(-1) d(-1)) and number of cycles (10) were observed in blend concentration of 1.0 g L(-1), renewal rate of 30%, and bicarbonate concentration of 1.0 g L(-1). The results showed the potential of semicontinuous cultivation of Cyanobium sp. in closed tubular bioreactor, combining factors such as blend concentration, renewal rate, and sodium bicarbonate concentration.
我们评估了在半连续模式下在垂直管式光生物反应器中生长的蓝藻 Cyanobium sp 的动力学培养特性。在 30°C、3200 Lux 和 12 h 光照/黑暗光周期下，在 2 L 的垂直管式光生物反应器中进行了为期 57 d 的培养。当培养物的混合浓度为 1.0 g L(-1)、更新率为 50%且碳酸氢钠浓度为 1.0 g L(-1)时，发现最大比生长速率为 0.127 d(-1)。在混合浓度为 1.0 g L(-1)、更新率为 30%且碳酸氢钠浓度为 1.0 g L(-1)时，观察到最大生产力（0.071 g L(-1) d(-1)）和循环次数（10）。结果表明，在封闭管式生物反应器中，通过结合混合浓度、更新率和碳酸氢钠浓度等因素，蓝藻 Cyanobium sp. 的半连续培养具有潜力。
