School of Biomolecular and Physical Sciences, Griffith University, Nathan 4111, Brisbane, QLD, Australia.
Biochem Genet. 2010 Apr;48(3-4):287-303. doi: 10.1007/s10528-009-9318-3. Epub 2009 Dec 24.
Mammalian ALDH3 isozymes participate in peroxidic and fatty aldehyde metabolism, and in anterior eye tissue UV-filtration. BLAT analyses were undertaken of the opossum genome using rat ALDH3A1, ALDH3A2, ALDH3B1, and ALDH3B2 amino acid sequences. Two predicted opossum ALDH3A1-like genes and an ALDH3A2-like gene were observed on chromosome 2, as well as an ALDH3B-like gene, which showed similar intron-exon boundaries with other mammalian ALDH3-like genes. Opossum ALDH3 subunit sequences and structures were highly conserved, including residues previously shown to be involved in catalysis and coenzyme binding for rat ALDH3A1. Eleven glycine residues were conserved for all of the opossum ALDH3-like sequences examined, including two glycine residues previously located within the stem of the rat ALDH3A1 active site funnel. Phylogeny studies of human, rat, opossum, and chicken ALDH3-like sequences indicated that the common ancestor for ALDH3A- and ALDH3B-like genes predates the appearance of birds during vertebrate evolution.
哺乳动物的 ALDH3 同工酶参与过氧化物和脂肪酸醛代谢，以及前眼部组织的紫外线过滤。使用大鼠 ALDH3A1、ALDH3A2、ALDH3B1 和 ALDH3B2 氨基酸序列对负鼠基因组进行了 BLAT 分析。在 2 号染色体上观察到两个预测的负鼠 ALDH3A1 样基因和一个 ALDH3A2 样基因，以及一个 ALDH3B 样基因，其内含子-外显子边界与其他哺乳动物的 ALDH3 样基因相似。负鼠 ALDH3 亚基序列和结构高度保守，包括先前证明参与大鼠 ALDH3A1 催化和辅酶结合的残基。在所检查的所有负鼠 ALDH3 样序列中，都保守了 11 个甘氨酸残基，包括两个先前位于大鼠 ALDH3A1 活性部位漏斗茎内的甘氨酸残基。人、大鼠、负鼠和鸡 ALDH3 样序列的系统发育研究表明，ALDH3A-和 ALDH3B 样基因的共同祖先先于脊椎动物进化过程中鸟类的出现。
