Unit of Aquaculture and Cryobiology, Department of Zoology, University of Madras, Guindy Campus, Chennai-600 025, India.
Ecotoxicol Environ Saf. 2011 May;74(4):623-9. doi: 10.1016/j.ecoenv.2010.08.027. Epub 2011 Feb 5.
In this investigation, effect of cadmium chloride (25 μg/l) on oogenesis of freshwater prawn, Macrobrachium rosenbergii was studied. In vivo experiments were performed with both intact and eyestalk ablated prawns. The intact, cadmium-exposed prawns exhibited decrease in Gonado Somatic Index (GSI) and ovarian development compared to controls. Whereas, ablated treated ovary showed reduction of yolk material and oocyte membrane thickness at the end of 15 days exposure. Interestingly, the control prawn showed normal cellular architecture of gills, hepatopancreas and ovary with mature oocytes. But, the gills of treated prawns showed lamellar hypertrophy, cuticular dystrophy and irregular arrangement of epithelial cells. Hepatopancreas showed reduction in both tubular diameter and basement membrane thickness. Conspicuously, ovary showed hypertrophied primary oocytes with more vacuoles in intact-treated group. Cadmium had increased gonad inhibiting hormone (GIH) secretion and decreased gonad stimulating hormone (GSH) release as evident with the retardation of gonadal maturation in the intact prawns.
本研究探讨了氯化镉（25μg/l）对罗氏沼虾（Macrobrachium rosenbergii）卵子发生的影响。采用完整和切除眼柄的虾进行体内实验。与对照组相比，暴露于镉的完整虾的性腺体指数（GSI）和卵巢发育均下降。而在 15 天暴露结束时，切除处理的卵巢显示卵黄物质减少和卵细胞膜厚度变薄。有趣的是，对照组虾的鳃、肝胰腺和卵巢具有成熟的卵子，呈现出正常的细胞结构。然而，处理虾的鳃显示出板层肥大、角质层营养不良和上皮细胞排列不规则。肝胰腺的管状直径和基膜厚度均减少。明显的是，在完整处理组中，卵巢显示出初级卵母细胞的肥大，并且有更多的空泡。镉增加了性腺抑制激素（GIH）的分泌，减少了性腺刺激激素（GSH）的释放，这与完整虾的性腺成熟延迟有关。
