Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture (Shanghai Ocean University), College of Fisheries and Life Science, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, No. 999 Hucheng Huan Road, Pudong New Area, Shanghai, 201306, China.
Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing, 210017, China.
Sci Rep. 2021 Aug 9;11(1):16140. doi: 10.1038/s41598-021-95709-w.
Heavy metal Cadmium (Cd) pollution has become a severe environmental problem for aquatic organisms. In crustaceans, gills (Gi) and hepatopancreas (Hp) play a vital role in the toxicology. However, in Macrobrachium rosenbergill, there are few researches about gill and hepatopancreases responding to Cd stress at a molecular level. In this study, transcriptomic analysis was applied to characterize gene expression profiles of gills and hepatopancreas of M. rosenbergill after Cd exposure for 0 h, 3 h and 3 d. Six cDNA libraries (Gi 0 h, Gi 3 h, Gi 3 d, Hp 0 h, Hp 3 h, and Hp 3 d) were constructed and a total of 66,676 transcripts and 48,991 unigenes were annotated. Furthermore, differentially expressed genes (DEGs) were isolated by comparing the Cd treated time-point libraries (3 h and 3 d group) with the control library (0 h group). The results showed that most of the DEGs were down-regulated after Cd exposure and the number of DEGs among gill groups were significantly higher than those among hepatopancreas groups. GO functional and KEGG pathway analysis suggested many key DEGs in response to the Cd stress, such as metallothionein and Hemocyanin. Additionally, a total of six DEGs were randomly selected to further identify their expressional profile by qPCR. The results indicated that these DEGs were involved in the response to Cd. This comparative transcriptome provides valuable molecular information on the mechanisms of responding to Cd stress in M. rosenbergii, which lays the foundation for further understanding of heavy metal stress.
重金属镉（Cd）污染已成为水生生物面临的严重环境问题。在甲壳动物中，鳃（Gi）和肝胰腺（Hp）在毒理学中起着至关重要的作用。然而，在罗氏沼虾中，关于鳃和肝胰腺对 Cd 胁迫的分子水平响应的研究较少。本研究应用转录组分析技术，研究了罗氏沼虾鳃和肝胰腺在 Cd 暴露 0 h、3 h 和 3 d 后的基因表达谱。构建了 6 个 cDNA 文库（Gi 0 h、Gi 3 h、Gi 3 d、Hp 0 h、Hp 3 h 和 Hp 3 d），共注释了 66676 个转录本和 48991 个 unigenes。此外，通过比较 Cd 处理时间点文库（3 h 和 3 d 组）与对照组文库（0 h 组），分离出差异表达基因（DEGs）。结果表明，Cd 暴露后大多数 DEGs 下调，且鳃组织中的 DEGs 数量明显高于肝胰腺组织中的 DEGs。GO 功能和 KEGG 通路分析表明，许多关键的 DEGs 参与了对 Cd 胁迫的响应，如金属硫蛋白和血蓝蛋白。此外，随机选择了 6 个 DEGs 通过 qPCR 进一步鉴定其表达谱。结果表明，这些 DEGs 参与了对 Cd 的响应。该比较转录组为罗氏沼虾对 Cd 胁迫的响应机制提供了有价值的分子信息，为进一步了解重金属胁迫奠定了基础。
