Academic Unit of Oral & Maxillofacial Medicine & Surgery, School of Clinical Dentistry, University of Sheffield, Sheffield S10 2TA, UK.
Microb Pathog. 2011 Jun;50(6):278-85. doi: 10.1016/j.micpath.2010.11.009. Epub 2011 Feb 3.
Candida albicans is a commensal organism that can be isolated from the majority of healthy individuals. However, in certain susceptible individuals C. albicans can become pathogenic leading to the mucocutaneous infection; oral candidiasis. Murine models and in vitro monolayer cultures have generated some data on the likely virulence and host factors that contribute to oral candidiasis but these models have limitations. Recently, tissue engineered oral mucosal models have been developed to mimic the normal oral mucosa but little information is available on their true representation. In this study, we assessed the histological features of three different tissue engineered oral mucosal models compared to the normal oral mucosa and analysed both cell damage and cytokine release following infection with C. albicans. Models comprised of normal oral keratinocytes and a fibroblast-containing matrix displayed more similar immunohistological and proliferation characteristics to normal mucosa, compared to models composed of an oral carcinoma cell line. Although all models were invaded and damaged by C. albicans in a similar manner, the cytokine response was much more pronounced in models containing normal keratinocytes. These data suggest that models based on normal keratinocytes atop a fibroblast-containing connective tissue will significantly aid in dissecting the molecular pathogenesis of oral candidiasis.
白色念珠菌是一种共生生物，可从大多数健康个体中分离出来。然而，在某些易感染的个体中，白色念珠菌可能会变得具有致病性，导致黏膜皮肤感染；口腔念珠菌病。鼠模型和体外单层培养已经产生了一些关于可能的毒力和宿主因素的数据，这些因素有助于口腔念珠菌病，但这些模型存在局限性。最近，组织工程口腔黏膜模型已经被开发出来，以模拟正常的口腔黏膜，但关于它们的真实表现的信息很少。在这项研究中，我们评估了三种不同的组织工程口腔黏膜模型与正常口腔黏膜的组织学特征，并分析了感染白色念珠菌后细胞损伤和细胞因子释放。与由口腔癌细胞系组成的模型相比，由正常口腔角质形成细胞和含有成纤维细胞的基质组成的模型显示出与正常黏膜更相似的免疫组织化学和增殖特征。尽管所有模型都以相似的方式被白色念珠菌侵袭和破坏，但含有正常角质形成细胞的模型中的细胞因子反应更为明显。这些数据表明，基于正常角质形成细胞和含有成纤维细胞的结缔组织的模型将极大地帮助剖析口腔念珠菌病的分子发病机制。
