Fukuma N, McLachlan S M, Petersen V B, Beever K, Smith B R
Department of Medicine, University of Wales College of Medicine, Cardiff, UK.
Autoimmunity. 1990;6(1-2):37-45. doi: 10.3109/08916939008993368.
The relationship between spectrotype and IgG subclass of autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) has been investigated using sera from Hashimoto and Graves' patients. Isoelectric focussing (IEF) was carried out in gels over the range pI 3.5-9.5 followed by transblotting to nitrocellulose and probing the filters using 125I-labelled TPO and Tg. As has been shown previously for Tg antibodies, TPO antibody focussed over different pI values in different patients but the spectrotypes for individuals were constant over 2-5 years. Further, the pI values for TPO and Tg autoantibodies appeared to be related to IgG subclass, for example IgG1 Tg antibodies tended to focus nearer the cathode (pI 8.0-9.5) than IgG4 antibodies (pI 6.5-8.5) while antibodies of subclasses IgG1 + 2 + 4 produced spectrotypes covering a broad pI range (5.7-9.5). Consequently, it seems likely that the characteristic spectrotypes described by others for Tg autoantibodies, and those we now report for TPO antibodies, reflect the IgG subclass "fingerprints" which we suggest may be a measure of the ability of an individual to respond to different epitopes on these two thyroid antigens.
利用桥本氏病患者和格雷夫斯病患者的血清，研究了甲状腺球蛋白（Tg）和甲状腺过氧化物酶（TPO）自身抗体的光谱类型与IgG亚类之间的关系。在pH值3.5 - 9.5范围内的凝胶中进行等电聚焦（IEF），随后转印至硝酸纤维素膜上，并用125I标记的TPO和Tg对滤膜进行检测。正如之前对Tg抗体的研究所示，TPO抗体在不同患者中聚焦于不同的pH值，但个体的光谱类型在2 - 5年内是恒定的。此外，TPO和Tg自身抗体的pH值似乎与IgG亚类有关，例如IgG1 Tg抗体往往比IgG4抗体（pH值6.5 - 8.5）更靠近阴极聚焦（pH值8.0 - 9.5），而IgG1 + 2 + 4亚类的抗体产生的光谱类型覆盖较宽的pH范围（5.7 - 9.5）。因此，其他人描述的Tg自身抗体的特征光谱类型以及我们现在报告的TPO抗体的光谱类型，似乎反映了IgG亚类的“指纹”，我们认为这可能是个体对这两种甲状腺抗原上不同表位反应能力的一种衡量。
