Kumar S Selva, Karrunakaran C M, Rao M R K, Balasubramanian M P
Department of Industrial Biotechnology, Bharath University, Chennai 600 073, India.
J Carcinog. 2011 Jan 11;10:1. doi: 10.4103/1477-3163.75458.
The anticancer and antioxidant effects of the aqueous extract of Indigofera aspalathoides on 20-methylcholanthrene (20-MCA) induced fibrosarcoma were investigated in male albino rats.
The rats were divided into four different groups, each group consisting of six animals. Group I animals were served as normal control, Group II animals were fibrosarcoma-bearing animals after the incubation period, Group III animals were fibrosarcoma-bearing animals, treated with aqueous extract of I. aspalathoides intraperitoneally at a dose of 250 mg/kg b.w. for 30 days and Group IV animals were administered with the aqueous extract of I. aspalathoides alone, at a dose of 250 mg/kg b.w. for 30 days, served as drug control animals. After the experimental period, all the rats were weighed and killed by cervical decapitation. The serum was separated from the blood for analysis. The weights of the liver and the kidneys were noted. The fibrosarcoma was proved by pathological examinations. The liver and kidney tissues were excised and then homogenized in an ice-cold buffer. These tissues were used for biochemical analysis.
The activities of antioxidant enzymes, e.g. catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), in blood serum, liver, and kidney of control and experimental animals, respectively, have been reported.
The present observations suggested that the aqueous extract of I. aspalathoides treatment enhanced the recovery from 20-MCA-induced fibrosarcoma due to its antioxidants and antineoplastic properties.
研究了靛蓝叶马棘水提取物对雄性白化大鼠20-甲基胆蒽（20-MCA）诱导的纤维肉瘤的抗癌和抗氧化作用。
将大鼠分为四组，每组六只动物。第一组动物作为正常对照，第二组动物为潜伏期后携带纤维肉瘤的动物，第三组动物为携带纤维肉瘤的动物，腹腔注射靛蓝叶马棘水提取物，剂量为250mg/kg体重，持续30天，第四组动物单独给予靛蓝叶马棘水提取物，剂量为250mg/kg体重，持续30天，作为药物对照动物。实验期结束后，称量所有大鼠体重并通过颈椎脱臼处死。分离血清进行分析。记录肝脏和肾脏的重量。通过病理检查证实纤维肉瘤。切除肝脏和肾脏组织，然后在冰冷缓冲液中匀浆。这些组织用于生化分析。
分别报告了对照组和实验组动物血清、肝脏和肾脏中抗氧化酶如过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性。
目前的观察结果表明，靛蓝叶马棘水提取物治疗可增强20-MCA诱导的纤维肉瘤的恢复，这归因于其抗氧化和抗肿瘤特性。
