Claimer C S, Mahesh A, Sinilal B, Rao D M, Thangadurai D
Department of Plant Science, Bharathidasan University, Tiruchirappalli-620 024, India.
Indian J Pharm Sci. 2012 Mar;74(2):157-60. doi: 10.4103/0250-474X.103851.
The chemopreventive effect of ethanol root extract of Indigofera aspalathoides was evaluated in N-nitrosodiethylamine-induced experimental liver tumor in mice. Pretreatment with ethanol root extract (100 mg/kg, p.o.) for three weeks significantly reduced the impact of N-nitrosodiethylamine toxicity (50 mg/kg, i.p.) on the serum markers of liver damage, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and total bilirubin. Protective effect was reconfirmed the elevated serum total protein levels were significantly restored towards normalization by the extracts, and this was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Histological examination of the liver of animals treated with ethanol root extract of Indigofera aspalathoides showed the reduction of necrosis. These results suggest that ethanol root extract of Indigofera aspalathoides possess protective effect against N-nitrosodiethylamine-induced hepatocellular carcinogenesis.
在N-亚硝基二乙胺诱导的小鼠实验性肝肿瘤中评估了天蓝木蓝乙醇根提取物的化学预防作用。用乙醇根提取物（100mg/kg，口服）预处理三周可显著降低N-亚硝基二乙胺毒性（50mg/kg，腹腔注射）对肝损伤血清标志物天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶和总胆红素的影响。提取物使升高的血清总蛋白水平显著恢复至正常，从而再次证实了保护作用，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳也证实了这一点。对经天蓝木蓝乙醇根提取物处理的动物肝脏进行组织学检查显示坏死减少。这些结果表明，天蓝木蓝乙醇根提取物对N-亚硝基二乙胺诱导的肝细胞癌具有保护作用。
