Center for Childhood Cancer, The Research Institute at Nationwide Children's Hospital, Columbus, Ohio, United States of America.
PLoS Genet. 2011 Jan 27;7(1):e1001282. doi: 10.1371/journal.pgen.1001282.
During cell division, the spindle checkpoint ensures accurate chromosome segregation by monitoring the kinetochore-microtubule interaction and delaying the onset of anaphase until each pair of sister chromosomes is properly attached to microtubules. The spindle checkpoint is deactivated as chromosomes start moving toward the spindles in anaphase, but the mechanisms by which this deactivation and adaptation to prolonged mitotic arrest occur remain obscure. Our results strongly suggest that Cdc28-mediated phosphorylation of Bub1 at T566 plays an important role for the degradation of Bub1 in anaphase, and the phosphorylation is required for adaptation of the spindle checkpoint to prolonged mitotic arrest.
在细胞分裂过程中，纺锤体检查点通过监测动粒-微管相互作用来确保染色体的正确分离，并延迟后期的开始，直到每对姐妹染色体都正确地附着在微管上。当染色体在后期开始向纺锤体移动时，纺锤体检查点就会失活，但这个失活和适应长时间有丝分裂阻滞的机制仍然不清楚。我们的结果强烈表明，CDC28 介导的 Bub1 在 T566 位的磷酸化对于后期中 Bub1 的降解起着重要作用，而且这种磷酸化对于纺锤体检查点适应长时间的有丝分裂阻滞是必需的。
