酵母有丝分裂纺锤体着丝粒的组成、功能与调控。
The composition, functions, and regulation of the budding yeast kinetochore.
机构信息
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
出版信息
Genetics. 2013 Aug;194(4):817-46. doi: 10.1534/genetics.112.145276.
Abstract
The propagation of all organisms depends on the accurate and orderly segregation of chromosomes in mitosis and meiosis. Budding yeast has long served as an outstanding model organism to identify the components and underlying mechanisms that regulate chromosome segregation. This review focuses on the kinetochore, the macromolecular protein complex that assembles on centromeric chromatin and maintains persistent load-bearing attachments to the dynamic tips of spindle microtubules. The kinetochore also serves as a regulatory hub for the spindle checkpoint, ensuring that cell cycle progression is coupled to the achievement of proper microtubule-kinetochore attachments. Progress in understanding the composition and overall architecture of the kinetochore, as well as its properties in making and regulating microtubule attachments and the spindle checkpoint, is discussed.
摘要
所有生物的繁殖都依赖于有丝分裂和减数分裂中染色体的准确有序分离。芽殖酵母长期以来一直是一种出色的模式生物，可用于鉴定调节染色体分离的成分和潜在机制。本综述重点介绍了动粒，这是一种组装在着丝粒染色质上的大型蛋白质复合物，可保持与纺锤体微管动态尖端的持续承重附着。动粒也是纺锤体检查点的调节中心，可确保细胞周期的进行与适当的微管-动粒附着相耦合。讨论了在理解动粒的组成和整体结构及其在制造和调节微管附着和纺锤体检查点方面的特性方面所取得的进展。
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