Grupo de Investigación Enzimología, Departamento de Bioquímica y Biología Molecular-A, Facultad de Biología, Universidad de Murcia, E-30100, Espinardo, Murcia, Spain.
J Enzyme Inhib Med Chem. 2011 Oct;26(5):728-33. doi: 10.3109/14756366.2010.548811. Epub 2011 Feb 8.
Tetrahydrobiopterin (BH(4)), methyl-tetrahydropterin (MBH(4)) and dimethyl-tetrahydropterin (DMBH(4)) are oxidized by tyrosinase in a process during which the suicide inactivation of tyrosinase may occur. From the kinetic study of this process, [Formula: see text] (apparent maximum constant for the suicide inactivation), [Formula: see text] (Michaelis constant for the substrate) and r (number of turnovers that the enzyme makes before the inactivation) can be obtained. From the results obtained, it can be deduced that the velocity of the inactivation governed by ([Formula: see text]) and the potency of the same ([Formula: see text]) follow the order: BH(4) > MBH(4) > DMBH(4).
四氢生物蝶呤（BH4）、甲基四氢生物蝶呤（MBH4）和二甲基四氢生物蝶呤（DMBH4）可被酪氨酸酶氧化，在此过程中，酪氨酸酶可能会发生自杀失活。通过对该过程的动力学研究，可以得到[公式：见文本]（自杀失活的表观最大常数）、[公式：见文本]（底物的米氏常数）和 r（酶失活前的周转率）。根据所得结果，可以推断失活速度受[公式：见文本]（）和相同的效力[公式：见文本]（）控制，遵循以下顺序：BH4＞MBH4＞DMBH4。
