Hanioka T, Amano A, Tamagawa H, Shizukuishi S
Osaka University Faculty of Dentistry.
J Osaka Univ Dent Sch. 1990 Dec;30:1-7.
Tissue reflectance spectrophotometry (TRS) can estimate hemoglobin concentration (Hb index) and oxygen saturation of hemoglobin (apparent SO2) in tissues. Reflectance spectra were measured in a gingiva-model and in human gingival biopsies by TRS. The gingiva-model was composed of human red cells and white gel in a cuvette placed on a black or white surface. The distance from the bottom surface of the cuvette to the optical probe was decreased from 5.0 to 0.5 mm. The Hb index in the gingiva-model on the black surface was constant before decreasing at less than a 3.0 mm distance. However, the Hb index in the gingiva-model on the white surface showed a decrease at less than a 1.5 mm distance. Apparent SO2 was constant at more than a 1.0 mm distance. Reflectance spectra were measured from human gingival tissue preparations, by slicing them off with microtome and making them less thick each time. Changes in reflectance spectra were observed in less than 1.0-1.5 mm thick specimens. These results suggest that the sensitivity in TRS measurement may exist within 1.0-1.5 mm from the gingival surface, and the limit of depth at which the TRS can measure may be within 3.0 mm.
组织反射分光光度法（TRS）可以估计组织中的血红蛋白浓度（Hb指数）和血红蛋白氧饱和度（表观SO2）。通过TRS在牙龈模型和人牙龈活检组织中测量反射光谱。牙龈模型由置于黑色或白色表面的比色皿中的人红细胞和白色凝胶组成。比色皿底面到光学探头的距离从5.0毫米减小到0.5毫米。在距离小于3.0毫米之前，黑色表面上牙龈模型中的Hb指数是恒定的。然而，白色表面上牙龈模型中的Hb指数在距离小于1.5毫米时显示下降。表观SO2在距离大于1.0毫米时是恒定的。通过用切片机将人牙龈组织切片并每次使其变薄来测量反射光谱。在厚度小于1.0 - 1.5毫米的标本中观察到反射光谱的变化。这些结果表明，TRS测量的敏感度可能存在于距牙龈表面1.0 - 1.5毫米范围内，并且TRS能够测量的深度极限可能在3.0毫米以内。
