硝酸盐同位素分析细菌方法的技术更新。

Technical updates to the bacterial method for nitrate isotopic analyses.

机构信息

Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.

出版信息

Anal Chem. 2011 Mar 1;83(5):1850-6. doi: 10.1021/ac1028984. Epub 2011 Feb 8.

DOI:10.1021/ac1028984

PMID:21302935

Abstract

The bacterial conversion of aqueous nitrate (NO(3)(-)) to nitrous oxide (N(2)O) for isotopic analysis has found widespread use since its introduction (Sigman, D. M.; Casciotti, K. L.; Andreani, M.; Galanter, M.; Böhlke, J. K. Anal. Chem.2001, 73, 4145-4153; Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905-4912). The bacterial strain Pseudomonas aureofaciens (ATTC no. 13985) was shown to convert NO(3)(-) to N(2)O while retaining both N and O isotopic signatures, and automation of the isotopic analysis of N(2)O greatly increased the throughput of the method (Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905-4912). Continued development of the denitrifier method has led to increased precision and throughput of NO(3)(-) isotopic analysis. Presented here are several recent procedural modifications and the demonstration of their effectiveness.

摘要

自引入以来，细菌将水合硝酸盐（NO3(-)）转化为氧化亚氮（N2O）以供同位素分析的方法得到了广泛应用（Sigman, D. M.; Casciotti, K. L.; Andreani, M.; Galanter, M.; Böhlke, J. K. Anal. Chem.2001, 73, 4145-4153; Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905-4912）。假单胞菌（Pseudomonas aureofaciens）（ATCC no. 13985）菌株被证明可以在保留氮和氧同位素特征的情况下将 NO3(-)转化为 N2O，并且 N2O 同位素分析的自动化大大提高了该方法的通量（Casciotti, K. L.; Sigman, D. M.; Galanter Hastings, M.; Böhlke, J. K.; Hilkert, A. Anal. Chem.2002, 74, 4905-4912）。不断发展的反硝化方法导致了 NO3(-)同位素分析的精度和通量的提高。本文介绍了几个最近的程序修改，并证明了它们的有效性。