采用高效液相色谱-蒸发光散射检测法同时测定台湾犁头尖根茎中的呋甾烷醇、波纳甾醇和薯蓣皂苷苷元糖苷。
Simultaneous Determination of Furostanol, Pennogenyl, and Diosgenyl Glycosides in Taiwanese Rhizoma Paridis ( Paris formosana Hayata) by high-performance liquid chromatography with evaporative light scattering detection.
机构信息
Department of Applied Chemistry, Chung Shan Medical University , 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan.
出版信息
J Agric Food Chem. 2011 Mar 9;59(5):1587-93. doi: 10.1021/jf104839r. Epub 2011 Feb 8.
A high-performance liquid chromatographic method with an evaporative light scattering detector (HPLC-ELSD) was developed to simultaneously determine 10 steroidal saponins, including 3 furostanol glycosides, 3 pennogenyl glycosides, and 4 diosgenyl glycosides in Taiwanese rhizoma paridis ( Paris formosana Hayata). The condition was a Cosmosil C18 column kept at 35 °C and a step-gradient solvent system consisting of acetonitrile and water (25:75, v/v) in the first 30 min, 45:55 (v/v) from 31 to 45 min, and 50:50 (v/v) from 45 to 65 min, at a flow rate of 1 mL/min. The separation factors (α) and resolutions (Rs) were better than 1, and the limits of detection (LODs) and limits of quantification (LOQs) were 0.01-0.27 and 0.04-0.90 μg, respectively, for these saponins. Moreover, 203 nm UV detection was also used for comparison. The saponins in P. formosana Hayata gathered from various areas of Taiwan were determined by applying the established method.
建立了一种高效液相色谱-蒸发光散射检测法（HPLC-ELSD），用于同时测定台湾白头翁根茎中的 10 种甾体皂苷，包括 3 种呋甾烷醇糖苷、3 种潘诺糖苷和 4 种薯蓣皂苷。条件为 Cosmosil C18 柱，保持在 35°C，采用梯度洗脱程序，流动相为乙腈-水（25:75，v/v）在最初的 30 min，31-45 min 时为 45:55（v/v），45-65 min 时为 50:50（v/v），流速为 1 mL/min。分离因子（α）和分辨率（Rs）均大于 1，这些皂苷的检测限（LOD）和定量限（LOQ）分别为 0.01-0.27 和 0.04-0.90 μg。此外，还使用 203 nm UV 检测进行了比较。应用所建立的方法对来自台湾不同地区的白头翁中的皂苷进行了测定。
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