反相高效液相色谱法测定重楼中甾体皂苷的含量

[Determination of steroidal saponins in Rhizoma paridis by RP-HPLC].

作者信息

Wei J

机构信息

Institute of Yunnan Baiyao Group Co. Ltd., Kunming 650032.

出版信息

Yao Xue Xue Bao. 1998 Jun;33(6):465-8.

PMID:12016919

Abstract

A reversed phase HPLC method for the separation and determination of nine steroidal saponins in Rhizoma paridis is described. The column was Symmetry C8, mobile phase was acetonitrile-water (42:58), UV detection was performed at 203 nm. The calibration curves showed good linearity over the range of 0.8-12.0 micrograms, gamma = 0.9996-0.9999, the recoveries were 97.1%-100.2% for the nine steroidal saponins. The method is simple, fast, sensitive and accurate and has been applied to the analysis of Rhizoma paridis. The results showed that the contents of the nine steroidal saponins in Rhizoma paridis were different with different sources.

摘要

描述了一种用于分离和测定重楼中9种甾体皂苷的反相高效液相色谱法。色谱柱为Symmetry C8，流动相为乙腈 - 水（42:58），于203 nm处进行紫外检测。校准曲线在0.8 - 12.0微克范围内呈良好线性，γ = 0.9996 - 0.9999，9种甾体皂苷的回收率为97.1% - 100.2%。该方法简便、快速、灵敏且准确，已应用于重楼的分析。结果表明，不同来源的重楼中9种甾体皂苷的含量不同。