Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
PLoS One. 2011 Feb 3;6(2):e16817. doi: 10.1371/journal.pone.0016817.
HCV transmission is closely associated with drug-trafficking routes in China. However, the transmission route of HCV in Eastern China remains unclear. Here, we investigate the role of Zhenjiang city of Jiangsu province, an important transportation hub linking Shanghai with other regions of China, in HCV transmission.
METHODOLOGY/PRINCIPAL FINDINGS: A total of 141 whole blood samples were collected from injection drug users (IDUs) in Zhenjiang and then tested for HCV infection. Of them, 115 HCV positive plasmas were subjected to RNA extraction, RT-PCR amplification, and sequencing. The subtype characterization and the evolutionary origin of HCV strains circulating in Zhenjiang were determined using polygenetic or phylogeographic analyses. Seven HCV subtypes 1b, 2a, 3a, 3b, 6a, 6e and 6n were detected among Zhenjiang IDUs, showing a complex HCV epidemic. The most predominant subtypes were 3a (38%) and 1b (26.8%). Among these subtypes, subtypes 3b, 6n and 6e originated from Southwestern China (i.e., Yunnan and/or Guangxi), subtypes 2a and 6a from Southern China (i.e., Guangdong), subtype 1b from Central (i.e., Henan) and Northwestern (i.e., Xinjiang) China, and subtype 3a from Southwestern (i.e., Yunnan) and Northwestern (i.e., Xinjiang) China. From Zhenjiang, subtypes 1b and 2a were further spread to Eastern (i.e., Shanghai) and Northern (i.e., Beijing) China, respectively.
CONCLUSIONS/SIGNIFICANCE: The mixing of seven HCV subtypes in Zhenjiang from all quarters of China indicates that as an important middle station, Zhenjiang plays a crucial role in HCV transmission, just as it is important in population migration between other regions of China and Eastern China.
在中国，HCV 的传播与贩毒途径密切相关。然而，华东地区 HCV 的传播途径尚不清楚。本研究调查了江苏省重要交通枢纽镇江市在 HCV 传播中的作用。
方法/主要发现：共采集镇江市 141 例静脉吸毒者（IDU）全血样本，检测 HCV 感染情况。其中，115 例 HCV 阳性血浆进行 RNA 提取、RT-PCR 扩增和测序。采用多基因或系统地理学分析确定镇江市流行的 HCV 毒株的亚型特征和进化起源。在镇江市 IDU 中检测到 7 种 HCV 亚型 1b、2a、3a、3b、6a、6e 和 6n，表明 HCV 流行复杂。最主要的亚型为 3a（38%）和 1b（26.8%）。在这些亚型中，亚型 3b、6n 和 6e 来源于中国西南部（即云南和/或广西），亚型 2a 和 6a 来源于中国南部（即广东），亚型 1b 来源于中国中部（即河南）和西北部（即新疆），亚型 3a 来源于中国西南部（即云南）和西北部（即新疆）。从镇江市传播的 1b 和 2a 亚型进一步传播到中国东部（即上海）和北部（即北京）。
结论/意义：镇江市来自全国各地的 7 种 HCV 亚型混合表明，作为一个重要的中间站，镇江市在 HCV 传播中发挥着关键作用，就像它在中国其他地区和华东地区之间的人口迁移中一样重要。
Zotero 插件
