Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China.
Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China.
J Med Virol. 2016 Mar;88(3):498-505. doi: 10.1002/jmv.24356. Epub 2015 Aug 31.
Hepatitis C virus (HCV) genotype distribution varied by regions and transmission modes. In this study, we investigated HCV genotype distribution in five cities of Jiangsu, China, all of which are located in the Yangtze River Delta Region. A total of 363 samples were collected during 2011-2012. C/E2 and NS5B fragments of HCV were amplified using a multiple RT-nested PCR strategy and subjected to sequencing. Phylogenetic analysis was performed for HCV genotyping. Among 106 PCR positive cases, HCV subtypes 1a (0.9%), 1b (61.3%), 2a (15.1%), 3a (4.7%), 3b (9.4%), 6a (6.6%), and 6n (1.9%) were detected. Together with our previous data, we found that HCV subtypes were more among injection drug users (IDUs) (nine) than among general population (GP) (six), and the most common subtype among GP was 1b (73.9%), followed by 2a (14.5%), while the top four common subtypes among IDUs were 3a, 1b, 3b, and 6a, with similar prevalence rates (24.4%, 22.7%, 20.9%, and 17.4%, respectively). There were nine HCV subtypes prevalent among IDUs in Jiangsu, more than those in Xinjiang, Hubei, Yunnan, Guangxi, Guangdong, and Hong Kong. The top four common subtypes among IDUs in Jiangsu covered all the two most common HCV subtypes (except 6n subtype) observed in six targeted provinces/region. These results suggested that Jiangsu may be an important gathering place for various HCV subtypes and the gathering may be involved in the large scale of population migration from other regions of China to Eastern China.
丙型肝炎病毒（HCV）基因型分布因地区和传播模式而异。本研究调查了中国江苏省五个城市的 HCV 基因型分布，这些城市均位于长江三角洲地区。2011 年至 2012 年期间共采集了 363 份样本。采用多重 RT-巢式 PCR 策略扩增 HCV 的 C/E2 和 NS5B 片段，并进行测序。对 HCV 基因分型进行了系统进化分析。在 106 例 PCR 阳性病例中，检测到 HCV 1a 亚型（0.9%）、1b 亚型（61.3%）、2a 亚型（15.1%）、3a 亚型（4.7%）、3b 亚型（9.4%）、6a 亚型（6.6%）和 6n 亚型（1.9%）。结合我们之前的数据，我们发现 HCV 亚型在注射吸毒者（IDUs）中更为常见（9 种），而在普通人群（GP）中则较少（6 种），在 GP 中最常见的亚型是 1b（73.9%），其次是 2a（14.5%），而 IDUs 中前四种常见亚型是 3a、1b、3b 和 6a，流行率相似（分别为 24.4%、22.7%、20.9%和 17.4%）。在江苏 IDUs 中流行的 HCV 亚型有 9 种，多于新疆、湖北、云南、广西、广东和香港。江苏 IDUs 中前四种常见亚型涵盖了在六个目标省份/地区观察到的两种最常见的 HCV 亚型（除 6n 亚型外）。这些结果表明，江苏可能是各种 HCV 亚型的重要聚集地，这种聚集可能涉及从中国其他地区到华东地区的大规模人口迁移。
