一种纯化脱氧胞苷激酶的通用方法。
A general method for purification of deoxycytidine kinase.
作者信息
Baxter A, Currie L M, Durham J P
出版信息
Biochem J. 1978 Sep 1;173(3):1005-8. doi: 10.1042/bj1731005.
Abstract
Deoxycytidine kinase from a variety of animal tissues binds to Cibacron Blue 3G-A coupled to Sepharose CL-6B. The enzyme can be selectively eluted with ATP to yield single-step purifications of 17-89-fold. The affinity adsorbent is re-usable.
摘要
来自多种动物组织的脱氧胞苷激酶能与偶联到琼脂糖凝胶CL-6B上的汽巴蓝3G-A结合。该酶可用ATP进行选择性洗脱，从而实现17至89倍的单步纯化。这种亲和吸附剂可重复使用。
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