Department of Diagnosis and Surgery, Faculdade de Odontologia de Araraquara-Univ Estadual Paulista (UNESP), Araraquara, SP, Brazil.
J Periodontal Res. 2011 Apr;46(2):269-79. doi: 10.1111/j.1600-0765.2010.01342.x. Epub 2011 Feb 8.
Curcumin is a plant-derived dietary spice with various biological activities, including anticarcinogenic and anti-inflammatory effects. Its therapeutic applications have been studied in a variety of conditions, including rheumatoid arthritis, colon cancer and depression, but no studies have evaluated the effects of curcumin on periodontal disease in vivo.
Experimental periodontal disease was induced in rats by placing cotton ligatures around both lower first molars. Curcumin was given to the rats by the intragastric route daily at two dosages (30 and 100 mg/kg) for 15 d. Control animals received ligatures but only the corn oil vehicle by gavage, and no treatment-negative control animals were included. Bone resorption was assessed by micro-computed tomography, and the inflammatory status was evaluated by stereometric analysis. Both RT-qPCR and ELISA were used to determine the expression of interleukin-6, tumor necrosis factor-α and prostaglandin E(2) synthase in the gingival tissues. Modulation of p38 MAPK and nuclear factor-κB activation were assessed by western blotting.
Bone resorption was effectively induced in the experimental period, but it was not affected by either dose of curcumin. Curcumin effectively inhibited cytokine gene expression at both the mRNA and the protein level and produced a dose-dependent inhibition of the activation of nuclear factor-κB in the gingival tissues. Activation of p38 MAPK was not inhibited by curcumin. Curcumin-treated animals also presented a marked reduction of the inflammatory cell infiltrate and increased collagen content and fibroblastic cell numbers.
Curcumin did not prevent alveolar bone resorption, but its potent anti-inflammatory effect suggests that it may have a therapeutic potential in periodontal diseases.
姜黄素是一种植物源性膳食香料，具有多种生物活性，包括抗癌和抗炎作用。其治疗应用已在多种疾病中进行了研究，包括类风湿性关节炎、结肠癌和抑郁症，但尚无研究评估姜黄素对体内牙周病的影响。
通过在下颌第一磨牙周围放置棉线结扎来诱导实验性牙周病。姜黄素通过灌胃以两种剂量（30 和 100 mg/kg）每天给予大鼠，共 15 天。对照动物仅接受结扎，但仅接受玉米油载体灌胃，且不包括无治疗阴性对照动物。通过微计算机断层扫描评估骨质吸收，通过体视学分析评估炎症状态。使用 RT-qPCR 和 ELISA 来确定牙龈组织中白细胞介素 6、肿瘤坏死因子-α和前列腺素 E2 合酶的表达。通过 Western 印迹来评估 p38 MAPK 和核因子-κB 的激活情况。
实验性牙周病有效诱导了骨质吸收，但姜黄素的两种剂量均未影响骨质吸收。姜黄素在 mRNA 和蛋白质水平上有效抑制细胞因子基因表达，并呈剂量依赖性抑制牙龈组织中核因子-κB 的激活。姜黄素未抑制 p38 MAPK 的激活。姜黄素处理的动物还表现出炎症细胞浸润的明显减少、胶原含量增加和成纤维细胞数量增加。
姜黄素不能预防牙槽骨吸收，但它具有强大的抗炎作用，表明它在牙周病中可能具有治疗潜力。
