Vantini G, Fusco M, Schiavo N, Gradkowska M, Zaremba M, Leon A, Oderfeld-Nowak B
CNS Department, Fidia Research Laboratories, Abano Terme, Italy.
Acta Neurobiol Exp (Wars). 1990;50(4-5):323-31.
The effect of nerve growth factor (NGF) on the intact septohippocampal cholinergic system of adult rats was studied. Nerve growth factor was continuously infused at different doses (5-100 micrograms) for two weeks into the lateral ventricle of adult rats. Controls received intracerebroventricular infusion of equal amounts of cytochrome c. Nerve growth factor treatment was capable of inducing a dose-dependent increase of choline acetyltransferase activity (ChAT) in septal area and ventral hippocampus. In both areas, the NGF-induced rise of ChAT activity was sustained for at least one week after infusion, then it progressively declined towards control values. By three and five weeks, using NGF at 25 and 100 micrograms respectively, ChAT increase was still significant in both septum and ventral hippocampus. The present findings corroborate a role for NGF in adult septohippocampal cholinergic system and indicate that the "pharmacological" modulation of these neurons by NGF may last several weeks following withdrawal of this trophic factor.
研究了神经生长因子（NGF）对成年大鼠完整的隔海马胆碱能系统的作用。将不同剂量（5 - 100微克）的神经生长因子连续两周注入成年大鼠的侧脑室。对照组接受脑室内注入等量的细胞色素c。神经生长因子治疗能够诱导隔区和腹侧海马中胆碱乙酰转移酶活性（ChAT）呈剂量依赖性增加。在这两个区域，NGF诱导的ChAT活性升高在注入后至少持续一周，然后逐渐下降至对照值。在分别使用25微克和100微克NGF的三周和五周时，隔区和腹侧海马中的ChAT增加仍然显著。目前的研究结果证实了NGF在成年隔海马胆碱能系统中的作用，并表明在撤除这种营养因子后，NGF对这些神经元的“药理学”调节可能持续数周。
