Schwaber J S, Due B R, Rogers W T, Junard E O, Sharma A, Hefti F
Neural Computation Group, E.I. duPont de Nemours, Wilmington, DE 19898.
J Comp Neurol. 1991 Jul 1;309(1):27-39. doi: 10.1002/cne.903090104.
The effectiveness of basic fibroblast growth factor and nerve growth factor in preventing the lesion-induced disappearance of septal cholinergic neurons was compared by using a computerized data-acquisition system and a digital brain atlas that yielded quantitative and distributional information. Adult rats were given unilateral partial transections of the fimbria and then received daily intraventricular injection of one of the growth factors for 15 days. Given the high degree of co-localization of nerve growth factor receptors with choline acetyltransferase in these areas, cholinergic neurons were identified by nerve growth factor receptor immunoreactivity. Their locations were plotted in the context of a three-dimensional brain atlas permitting the analysis of relative distributions of cholinergic neurons in control brains and those of animals treated with each growth factor. The cholinergic cell disappearance induced by the partial fimbrial transection was restricted to the medial septal nucleus and the vertical limb of the diagonal band of Broca. Within the affected areas cholinergic cell disappearance increased gradually in severity from anterior to posterior levels of the septal nucleus. Both growth factors prevented the disappearance of cholinergic cell bodies in medial septal nucleus and vertical limb of the diagonal band. In lesioned control animals the unilateral cell disappearance amounted to 53.5% of the number of cholinergic neurons of the unlesioned side. Nerve growth factor and basic fibroblast growth factor reduced this disappearance to 13% and 28%, respectively. The distribution of cholinergic cells was the same in animal treated with each growth factor, suggesting that the two growth factors protect the same population of cholinergic neurons.
通过使用计算机数据采集系统和数字脑图谱（可提供定量和分布信息），比较了碱性成纤维细胞生长因子和神经生长因子在预防损伤诱导的隔区胆碱能神经元消失方面的有效性。成年大鼠接受单侧穹窿部分横断，然后每天脑室内注射一种生长因子，持续15天。鉴于这些区域神经生长因子受体与胆碱乙酰转移酶高度共定位，通过神经生长因子受体免疫反应性鉴定胆碱能神经元。在三维脑图谱的背景下绘制它们的位置，以便分析对照脑以及用每种生长因子处理的动物脑中胆碱能神经元的相对分布。部分穹窿横断诱导的胆碱能细胞消失局限于内侧隔核和布罗卡斜带垂直支。在受影响区域内，胆碱能细胞消失的严重程度从隔核的前部到后部逐渐增加。两种生长因子均能防止内侧隔核和斜带垂直支中胆碱能细胞体的消失。在损伤对照动物中，单侧细胞消失量占未损伤侧胆碱能神经元数量的53.5%。神经生长因子和碱性成纤维细胞生长因子分别将这种消失减少到13%和28%。用每种生长因子处理的动物中胆碱能细胞的分布相同，表明这两种生长因子保护相同群体的胆碱能神经元。
