Cystic fibrosis marker testing in Bohemia with polymerase chain reaction.
作者信息
Macek M, Böhm I, Arnold L, Smrt J, Duspivová R, Vávrová V, Macek M, Jelínková E, Schmidtke J
机构信息
I. Institute for Human Genetics, Free University, West Berlin.
出版信息
Acta Univ Carol Med (Praha). 1990;36(1-4):108-11.
PMID:2130670
Abstract
Conditions for assessing KM-19 probe detected by Pst-1 restriction fragment length polymorphism (RFLP) by means of polymerase chain reaction were provided. Computer controlled mechanical arm with waterbaths and cloned heat-stable DNA polymerase was used. Results of KM-19 allelic frequencies on 90 cystic fibrosis chromosomes are presented. Allele two frequency was -0.833.
摘要
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